Kinetex biphenyl c18 column

Chromatographic analysis was performed on a SCIEX Exion LC TM ultra-high performance liquid chromatography (UHPLC) System with a Phenomenex Kinetex® Biphenyl C18 column (2. 6 μm, 100 x 2.1 mm). A gradient elution using 0.1% formic in water as solvent A and acetonitrile as solvent B was performed with a flow rate of 0.3 mL/min over 25 minutes. The gradient elution process was shown in Table 1. The column temperature was set at 40 and the inject volume was 2 μL. A TripleTOF® 5600+ system with a DuoSpray Source (electrospray ionization) was used for data acquisition in positive mode over a mass range of 100 -1250 m/z. Automated calibration was performed using an external calibrant delivery system (CDS) which infuses calibration solution prior to sample introduction. A TOF MS survey scan experiment (100 ms) incorporating IDA set to monitor ten candidate ions (total cycle time of 350 ms) was performed with a collision energy of 35 eV and a spread of ±15 eV. The optimal conditions were set as follows: Ion source gas 1 (GS1), 55 psi; Ion source gas 2 (GS2), 55 psi; curtain gas (CUR), 35 psi; spray voltage, 5.5 kV (positive ESI mode); source heater temperature, 550 °C. Data were processed using Analyst TF 1.8.1 software. For features of interest, the MS and MS/MS data were further evaluated using Formula Finder and the structural elucidation tool within SCIEX OS Software.