Log In Sign up
The largest database of trusted experimental protocols

Anti importin 7

Manufactured by Abcam
Visit Supplier

Anti-importin 7 is a primary antibody that recognizes the importin 7 protein. Importin 7 is a nuclear transport receptor involved in the trafficking of proteins into the cell nucleus.

Automatically generated - may contain errors

Lab products found in correlation

Anti gapdh, by Santa Cruz Biotechnology (2 mentions) Anti importin β1, by Santa Cruz Biotechnology (2 mentions) Horseradish peroxidase hrp conjugated donkey anti rabbit igg, by GE Healthcare (2 mentions) Anti importin 8, by Abcam (2 mentions) Gfp trap a, by Proteintech (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Ponceau s solution, by Merck Group (1 mentions)

3 protocols using anti importin 7

1

Immunoblotting Analysis of Importin Proteins in HeLa Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Twenty six hours post-transfection, HeLa cell protein lysates were prepared and analyzed by immunoblotting as described (Subramanian et al., 2015 (link)). Antibodies were used with the following concentrations: anti-GAPDH (Santa Cruz Biotechnology Inc, Dallas, TX), 1:5000; anti-importin β1 (Santa Cruz), 1:2000; anti-importin 4 (Santa Cruz), 1:333; anti-importin 5 (Santa Cruz), 1:10,000; anti-importin 7 (Abcam, Cambridge, MA), 1:1000; anti-importin 8 (Abcam), 1:250; anti-importin 9 (Abcam), 1:250; anti-importin 11 (Abcam), 1:333; anti-importin 13 (Santa Cruz), 1:100; anti-importin α1 (Santa Cruz), 1:2000; anti-importin α3 (Thermo Scientific), 0.2 μg/ml; horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG (GE Healthcare Life Sciences), 1:25,000; HRP-sheep anti-mouse IgG (GE Healthcare Life Sciences), 1:25,000; or HRP-mouse anti-goat IgG (Santa Cruz Biotechnology), 1:25,000. Protein size was confirmed using Pre-Stained Kaleidoscope Protein Standards (Bio-Rad, Hercules, CA). X-ray films were quantified by scanning densitometry using NIH ImageJ software.
Roggero V.R., Zhang J., Parente L.E., Doshi Y., Dziedzic R.C., McGregor E.L., Varjabedian A.D., Schad S.E., Bondzi C, & Allison L.A. (2015). Nuclear Import of the Thyroid Hormone Receptor α1 is Mediated by Importin 7, Importin β1, and Adaptor Importin α1. Molecular and cellular endocrinology, 419, 185-197.
+ Open protocol
+ Expand
2

Immunoprecipitation of GFP-Interacting Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were seeded in 100 mm vented plates at a concentration of 11
× 105 cells per plate in MEM supplemented with 10% FBS.
Twenty-four hours post-seeding, each plate was transfected with the desired
expression plasmids using Lipofectamine 2000. After 26 h, GFP-Trap®_A
(Chromotek) coimmunoprecipitation assays were performed as described (Roggero et al., 2016 (link)). Samples of unbound
and bound proteins were analyzed by immunoblotting. Membranes were stained for
total protein with Ponceau S solution (MilliporeSigma), quantified by scanning
densitometry using NIH ImageJ software, then destained prior to application of
antibodies at the following concentrations: anti-GFP (Santa Cruz), 1:2000;
anti-importin 7 (Abcam), 1:1000; anti-importin (karyopherin) β1 (Santa
Cruz), 1:1000; anti-importin α1 (Abcam), 1:1000; horseradish peroxidase
(HRP)-conjugated donkey anti-rabbit IgG (GE Healthcare Life Sciences); or
HRP-sheep anti-mouse IgG (Santa Cruz). Protein size was confirmed using
Pre-Stained Kaleidoscope Protein Standards (Bio-Rad). X-ray films were
quantified by scanning densitometry using NIH ImageJ software.
Anyetei-Anum C.S., Evans R.M., Back A.M., Roggero V.R, & Allison L.A. (2019). Acetylation modulates thyroid hormone receptor intracellular localization and intranuclear mobility. Molecular and cellular endocrinology, 495, 110509.
+ Open protocol
+ Expand
3

Importin Knockdown and Subcellular Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Knockdown of importin β1, importin 7, and importin 8, compared to scrambled control shRNA, and protein compartment analysis was done by western blot as previously described 25 . Briefly, 786-0 cells in 6-well plates were transfected with sets of four shRNA plasmids (SABioscience, Frederick, MD) and lysed 24 hours post-transfection. Antibodies were used with the following concentrations: anti-GAPDH (Santa Cruz Biotechnology Inc, Dallas, TX), 1:5000; anti-importin β1 (Santa Cruz), 1:2000; anti-importin 7, 1:1000; anti-importin 8, 1:250 (both Abcam, Cambridge, MA). Independent replicates of each importin knockdown were completed from different cultures across different days.
Rosenberg D., Kolla L., Heo D., Cassio E., Veenstra M.J., Vakaki M.A., Zhang J., Anyetei‐Anum C.S., Allison L.A., & Buchser W. (2020). Modulating Intra-Nuclear LC3 with Small Molecules Rescues Cells from a Docetaxel-Induced Phenotype.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!