The breast cancer cell lines BT-20, BT-549, EFM-19, MCF-7, MDA-MB-157, MDA-MB-231, MDA-MB-361, MDA-MB-436, MDA-MB-468, T-47D were purchased from ATCC. NCI-H1703 (KRAS mutant vs wild-type), DLD-1 (KRAS G13D/wt) and DWT7 (del/wt) were previously described [16 (link)]. MCF-7, MDA-MB-157, MDA-MB-231, MDA-MB-361, MDA-MB-436, MDA-MB-468 were cultivated in DMEM, BT-549, EFM-19, T-47D, NCI-H1703 in RPMI, DLD-1 and DWT7 in McCoy media, and BT-20 in Eagle’s Minimum Essential Medium, supplemented with 10% BGS, 2mM glutamine, 100 U/ml penicillin and 10% HEPES (all Sigma-Aldrich). All cell lines were cultured in a humidified incubator at 37°C and 5% CO2 and passaged for < 3 months after thawing a given frozen vial. All cell lines were tested mycoplasma free prior to the experiments (MycoAlert, Lonza) and none was ever treated for mycoplasma throughout the experiments. For 3D culture of tumor spheres, ~10,000 cells/well were grown in black round bottom polystyrene ultra-low attachment microplates (Corning) using serum-free medium composed of DMEM (Sigma-Aldrich), basic fibroblast growth factor (bFGF), and EGF (20 ng/mL each, Sigma-Aldrich), and B27 supplement (Life Technologies).
Bt 20
Manufactured by Merck Group
Sourced in United States
The BT-20 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use, but a detailed description of its core function is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 468, by Merck Group (6 mentions)
Mda mb 231, by Merck Group (6 mentions)
Mda mb 231, by American Type Culture Collection (6 mentions)
Bt 20, by American Type Culture Collection (6 mentions)
Mda mb 468, by American Type Culture Collection (5 mentions)
Bt 549, by Merck Group (4 mentions)
Mcf 7, by Merck Group (4 mentions)
Mda mb 436, by Merck Group (3 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (3 mentions)
Sk br 3, by Merck Group (3 mentions)
Mcf 7, by American Type Culture Collection (3 mentions)
Bt549, by American Type Culture Collection (3 mentions)
Mda mb 436, by American Type Culture Collection (3 mentions)
Mda mb 157, by Merck Group (2 mentions)
Hs578t, by Merck Group (2 mentions)
Roswell park memorial institute (rpmi), by Merck Group (2 mentions)
Hcc1937, by Merck Group (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
Hek293, by Merck Group (2 mentions)
Hek293, by American Type Culture Collection (2 mentions)
8 protocols using bt 20
1
Culturing Breast Cancer Cell Lines
2
Breast Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BC cell lines were purchased from ATCC (VA, USA) (Additional file 1 : Table S1). MCF7, T47D, BT-549 and MDA-MB-231 were cultured in RPMI (Sigma Aldrich, USA), while BT-474, SkBr3, MDA-MB-468, BT-20 and Hs578T were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma Aldrich, USA). The immortalized human mammary epithelial cells hTERT-HME1 (ATCC, USA) were grown in DMEM/F-12 mixture medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma Aldrich, USA). The human mammary epithelial cells HMEpC were purchased from cell applications (CA, USA) and maintained in defined mammary epithelial cell medium provided by the company (Cell applications, USA).
3
Cell Line Characterization and Maintenance
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human epithelial cell lines were purchased from ATCC: HeLa (CCL-2), T47D (HTB-133) and BT20 (HTB-19). Cells were authenticated in 2019 through short tandem repeat (STA) analysis of 21 markers by Eurofins Genomics with positive results, checked monthly for mycoplasma via a PCR-based detection assay (Venor®GeM – Cambio), and grown in the indicated media supplemented with 2 mM l -glutamine and 100 U/ml penicillin, 100 μg/ml streptomycin and 10% foetal bovine serum. HeLa, BT20 and Lenti-XL cells were grown in StableCell™ DMEM–- high glucose (Sigma-Aldrich). T47D were grown in RPMI 1640 Medium, GlutaMAX™ Supplement (Gibco).
4
Culturing Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-231, BT-549, BT-20, HCC1937, MCF-7, T-47D, ZR-75-1, SK-BR-3 cell lines were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). All cell lines were grown in monolayers in appropriate media supplemented with 10% fetal bovine serum and 1% antibiotic/antimycotic solution (Sigma-Aldrich): RPMI 1640 Medium for BT-549, T-47D, HCC1937 and ZR-75-1 cells; Minimum Essential Medium for MCF-7 and BT-20 cells; McCoy’s 5A Medium for SK-BR-3 cells; Leibovitz’s L-15 Medium for MDA-MB-231 cells. All cells were maintained at 37°C in humid air with 5% CO2 condition (BT-549, T-47D, HCC1937, ZR-75-1, MCF-7, BT-20 and SK-BR-3), or without CO2 (MDA-MB-231). All cell lines were authenticated by STR analysis.
5
Characterization of TNBC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TNBC cell lines BT20, CAL51, HCC70, HCC1143, HCC1187, HCC1806, HCC1937, Hs578T,
MDA-MB-157, MDA-MB-231 and MDA-MB-468 were obtained from the American Tissue
Culture Collection (Rockville, MD, USA). TNBC cell lines CAL120, CAL851 and
HDQP1 were obtained from the German Tissue Repository DMSZ (Braunschweig,
Germany). All cell lines were tested for mycoplasma and authenticated by short
tandem repeat (STR) typing (Additional File 1). The HCC1143, HCC1187, HCC1806,
HCC1937, Hs578T, MDA-MB-231 and MDA-MB-468 cells were cultured in RPMI
(Sigma-Aldrich) containing 10% foetal calf serum (FCS; Life Technologies); the
HCC70 cells were cultured in RPMI containing 10% FCS, 1 mM sodium pyruvate (Life
Technologies) and 2 mM nonessential amino acids (Life Technologies); the HDQP1
cells were cultured in DMEM (Sigma-Aldrich) containing 10% FCS; the CAL51 cells
were cultured in DMEM containing 10% FCS and 1 mM sodium pyruvate; the CAL120
and CAL851 cells were cultured in DMEM (Sigma-Aldrich) containing 10% FCS, 1 mM
sodium pyruvate and 2 mM glutamine (Life Technologies); the BT20 cells were
cultured in DMEM-HAM F12 (Sigma-Aldrich) containing 10% FCS; the MDA-MB-157
cells were cultured in Leibovitz L15 (Sigma-Aldrich) containing 10% FCS. Cells
were incubated at 37°C and 5% CO2.
MDA-MB-157, MDA-MB-231 and MDA-MB-468 were obtained from the American Tissue
Culture Collection (Rockville, MD, USA). TNBC cell lines CAL120, CAL851 and
HDQP1 were obtained from the German Tissue Repository DMSZ (Braunschweig,
Germany). All cell lines were tested for mycoplasma and authenticated by short
tandem repeat (STR) typing (Additional File 1). The HCC1143, HCC1187, HCC1806,
HCC1937, Hs578T, MDA-MB-231 and MDA-MB-468 cells were cultured in RPMI
(Sigma-Aldrich) containing 10% foetal calf serum (FCS; Life Technologies); the
HCC70 cells were cultured in RPMI containing 10% FCS, 1 mM sodium pyruvate (Life
Technologies) and 2 mM nonessential amino acids (Life Technologies); the HDQP1
cells were cultured in DMEM (Sigma-Aldrich) containing 10% FCS; the CAL51 cells
were cultured in DMEM containing 10% FCS and 1 mM sodium pyruvate; the CAL120
and CAL851 cells were cultured in DMEM (Sigma-Aldrich) containing 10% FCS, 1 mM
sodium pyruvate and 2 mM glutamine (Life Technologies); the BT20 cells were
cultured in DMEM-HAM F12 (Sigma-Aldrich) containing 10% FCS; the MDA-MB-157
cells were cultured in Leibovitz L15 (Sigma-Aldrich) containing 10% FCS. Cells
were incubated at 37°C and 5% CO2.
6
Cell Culture Maintenance of Breast Cancer Lines
7
Cell Culture Conditions for Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human breast cancer cells MDA-MB 468, BT20, MDA-MB 231, MCF7, T47D, SK-BR-3, HEK-293 and mouse 4T1 breast cancer cells were obtained from the American Type Culture Collection. MDA-MB 468 and HEK-293 cells were maintained in DMEM (Sigma) supplemented with 2 μM L-glutamine (Sigma); BT20 cells were maintained in EMEM (Sigma) supplemented with 1 mM Sodium Pyurvate (Sigma); MDA-MB 231, MCF7, T47D, 4T1 and PBMCs were maintained in RPMI 1640 cell culture media (Sigma); SK-BR-3 cells were maintained in DMEM/F12 cell culture media (Sigma). All culture media were supplemented with 10% FBS and 1% Penicillin-Streptomycin (P+S) and cultures were grown at 37 °C with 5% CO2.
8
Culturing Mammary and Kidney Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human mammary epithelial cell line MCF-10A and TNBC cell lines MDA-MB-436, MDA-MB-231, MDA-MB-468, BT-549, BT-20 and Human Embryonic Kidney 293 (HEK293) cells were purchased from the American Type Culture Collection (Manassas, VA). MDA-MB-436, MDA-MB-231, MDA-MB-468, BT-549, BT-20 and HEK293 cells were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 10% FBS and a 100-U/ml penicillin-streptomycin solution (Sigma). MCF-10A cells were maintained in a nutrient mixture consisting of DMEM/F12 supplemented with 5% horse serum, epidermal growth factor, hydrocortisone, insulin and cholera toxin. All cultured cells were incubated at 37°C in a water-saturated 95% air–5% CO2 atmosphere.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!