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High phosphate diet

Manufactured by Altromin
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Sourced in Germany

The high-phosphate diet is a laboratory equipment product designed to provide a controlled dietary environment for research purposes. The product offers a precise formulation with a high concentration of phosphate, a key mineral essential for various physiological processes. This diet can be utilized in scientific studies to investigate the effects of elevated phosphate levels on biological systems.

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Lab products found in correlation

Dimethyl sulphoxide, by Merck Group (2 mentions) R 7050, by Santa Cruz Biotechnology (2 mentions) Dba 2, by Charles River Laboratories (2 mentions) Etanercept, by Pfizer (2 mentions)

2 protocols using high phosphate diet

1

Murine Model of Vascular Calcification

Cited 1 time
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Female 8-week-old dilute-brown agouti 2 (DBA/2NCrl, hereafter referred to as DBA/2) mice were obtained from Charles River (Sulzfeld, Germany) and housed in a virus/antibody-free environment. These mice have an inherent susceptibility to high-phosphate diet-triggered calcification [15 (link), 16 (link)]. To induce media calcification, they were placed on high-phosphate diet (Altromin, Germany) containing 20.2 g phosphorus, 9.4 g calcium, 0.7 g magnesium and 500 IU vitamin D3 per kg. The standard chow contained 7.0 g phosphorus, 10.0 g calcium, 2.2 g magnesium and 1000 IU vitamin D3 per kg. Mice were then followed for 5–14 days and culled under anaesthesia. For the interventional studies, DBA/2 mice were divided into three treatment groups to receive vehicle control (dimethylsulphoxide; Sigma, St. Louis, MO), TNFα inhibitor etanercept (Pfizer, New York, NY) at a dose of 10 mg/kg body weight, or TNFα receptor antagonist R-7050 (Santa Cruz, Dallas, TX) at a dose of 6 mg/kg body weight, respectively [17 (link)]. These drugs were applied via intraperitoneal injections every alternate day. All animal experiments were approved by Austrian veterinary authorities (BMWF-66.010/0047-II/3b/2012) and corresponded to directive 2010/63/EU of the European Parliament.
Kirsch A.H., Kirsch A., Artinger K., Schabhüttl C., Goessler W., Klymiuk I., Gülly C., Fritz G.A., Frank S., Wimmer R., Brodmann M., Anders H.J., Pramstaller P.P., Rosenkranz A.R., Eller K, & Eller P. (2015). Heterogeneous susceptibility for uraemic media calcification and concomitant inflammation within the arterial tree. Nephrology Dialysis Transplantation, 30(12), 1995-2005.
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2

DBA/2 Mouse Model of Vascular Calcification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Female 8-week-old dilute-brown agouti 2 (DBA/2NCrl, hereafter referred to as DBA/2) mice were obtained from Charles River (Sulzfeld, Germany) and housed in a virus/antibody-free environment. These mice have an inherent susceptibility to high-phosphate diet-triggered calcification [15 (link),16 (link)]. To induce media calcification, they were placed on high-phosphate diet (Altromin, Germany) containing 20.2 g phosphorus, 9.4 g calcium, 0.7 g magnesium and 500 IU vitamin D3 per kg. The standard chow contained 7.0 g phosphorus, 10.0 g calcium, 2.2 g magnesium and 1000 IU vitamin D3 per kg. Mice were then followed for 5–14 days and culled under anaesthesia. For the interventional studies, DBA/2 mice were divided into three treatment groups to receive vehicle control (dimethylsulphoxide; Sigma, St. Louis, MO), TNFα inhibitor etanercept (Pfizer, New York, NY) at a dose of 10 mg/kg body weight, or TNFα receptor antagonist R-7050 (Santa Cruz, Dallas, TX) at a dose of 6 mg/kg body weight, respectively [17 (link)]. These drugs were applied via intraperitoneal injections every alternate day. All animal experiments were approved by Austrian veterinary authorities (BMWF-66.010/0047-II/3b/2012) and corresponded to directive 2010/63/EU of the European Parliament.
Kirsch A.H., Kirsch A., Artinger K., Schabhüttl C., Goessler W., Klymiuk I., Gülly C., Fritz G.A., Frank S., Wimmer R., Brodmann M., Anders H.J., Pramstaller P.P., Rosenkranz A.R., Eller K, & Eller P. (2015). Heterogeneous susceptibility for uraemic media calcification and concomitant inflammation within the arterial tree. Nephrology Dialysis Transplantation, 30(12), 1995-2005.
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