The following antibodies were used: rat anti-CD300f monoclonal antibody (3-14-11; rat IgG2a) (ActGen); FITC-conjugated CD11b (M1/70), F4/80 (BM8), FcϵRIα (MAR-1), and Ly5.1(A20), phycoerythrin (PE)-conjugated Gr-1 (RB6-8C5), CD11b (M1/70), c-Kit (2B8), Ly5.2 (104), and rat IgG2a (eBR2a) (eBioscience); PE-conjugated Ly-6G (1A8) and streptavidin-allophycocyanin (APC) (BioLegend); anti-ceramide (MID 15B4) (Enzo Life Sciences); and anti-mouse IgM (MOPC-104E) (BioLegend). Cytokines were obtained from R&D Systems, and C-24 ceramide was obtained from Toronto Research Chemicals, Inc. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (PC) was obtained from Echelon Biosciences Inc.
Anti ceramide mid 15b4
Manufactured by Enzo Life Sciences
Anti-ceramide (MID 15B4) is a laboratory reagent used in research applications. It is an antibody that specifically binds to the lipid molecule ceramide, allowing for the detection and study of ceramide levels in biological samples.
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2 protocols using anti ceramide mid 15b4
1
Comprehensive Immune Cell Phenotyping
2
Antibody Characterization for Immune Cell Analysis
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The following antibodies (Abs) were used: biotinylated anti-LMIR3/CD300f (3-14-11; rat IgG2a) (ACTGen, Nagano, Japan), fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD3ε, anti-mouse/human B220, and anti-mouse Ly-6G, anti-mouse CD4, anti-mouse CD8a, anti-mouse CD11c, anti-mouse CD19, peridinin chlorophyll protein (PerCP)-conjugated anti-mouse/human CD11b, Brilliant Violet 421-conjugated anti-mouse CD11c, phycoerythrin (PE)/cyanine 7 (Cy7)-conjugated anti-mouse FcεRI (MAR-1), PerCP-Cy5.5-conjugated anti-mouse CD49b, allophycocyanin (APC)-conjugated anti-mouse c-Kit and mouse Ly-6G, APC/Cy7-conjugated anti-mouse CD45, and Brilliant Violet 450-conjugated anti-mouse CD45 (BioLegend, San Diego, CA), and anti-ceramide (MID 15B4; Enzo Life Sciences, Plymouth Meeting, PA). Mouse IgM (MOPC-104E; BioLegend, San Diego, CA) was used as a control for anti-ceramide Ab. PE-conjugated streptavidin was purchased from BioLegend. RW pollen and RW extract were purchased from PolyScience (Niles, IL) and LSL Co Ltd (Tokyo, Japan), respectively. Cytokines were obtained from R&D Systems (Minneapolis, MN). C-24 ceramide [ceramide (d18:1/24:0); C42H83NO3] was obtained from Toronto Research Chemicals Inc. (Toronto, Canada). Phosphatidylserine (PS) [1,2-Dipalmitoyl-sn-glycero-3-phosphoserine] was obtained from Echelon Biosciences Inc. (Salt Lake City, UT).
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