Zen blue 3.1 pro software

All live-cell imaging experiments were performed on an inverted widefield epi-fluorescence microscope Zeiss Axio Observer.Z1/7 (Carl Zeiss AG, Oberkochen, Germany) equipped with an LED light source Colibri 7 (423/44 nm, 469/38 nm, 555/30), Plan-Apochromat 20×/0.8 dry objective, Plan-Apochromat 40×/1.4 oil immersion objective, a monochrome CCD camera Axiocam 503, and a custom-made gravity-based perfusion system. HyPer7 signals were imaged by alternately exciting cells using a motorized dual-filter wheel equipped with beam splitters (FT455 (for HyPer low, F₄₂₀) and FT495 (for HyPer high, F₄₉₀)). Emissions were alternately collected using a bandpass filter (BP 525/50). HyPerRed emission was collected using the filter combinations FT570 (BS) and emission filter 605/70. During the experiments, control and data acquisition were executed using Zen Blue 3.1 Pro software (Carl Zeiss AG, Oberkochen, Germany). Administration and withdrawal of exogenous H₂O₂ were performed using a custom-made perfusion system connected to a metal perfusion chamber (NGFI, Graz, Austria).

Live-cell imaging experiments were performed on an inverted wide-field epi-fluorescence microscope Zeiss Axio Observer.Z1/7 (Carl Zeiss AG, Oberkochen, Germany) equipped with an LED light source Colibri 7 (423/44 nm, 469/38 nm, 555/30), Plan-Apochromat 20x/0.8 dry objective, Plan-Apochromat 40x/1.4 oil immersion objective, a monochrome C.C.D. camera Axiocam 503, and a custom-made gravity-based perfusion system. O-geNOps signals were imaged by exciting cells (555 nm) using a motorized dual filter wheel equipped with the filter combinations FT570 (BS) and emission filter 605/70. Control and data acquisition were executed using Zen Blue 3.1 Pro software (Carl Zeiss AG, Oberkochen, Germany). Administration and withdrawal of 3-(2-Hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-propanamine (NOC-7) were performed using a custom-made perfusion system connected to a metal perfusion chamber (NGFI, Graz, Austria).