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Thruplex dnaseq sample preparation kits

Manufactured by Takara Bio
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ThruPLEX-DNAseq sample preparation kits are a set of reagents and protocols designed for the preparation of DNA samples for next-generation sequencing. The kits facilitate the conversion of DNA samples into sequencing-ready libraries.

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Lab products found in correlation

Agilent 2200 tapestation, by Agilent Technologies (2 mentions) Kapa library quantification kit, by Roche (2 mentions) Nextseq500 run, by Illumina (2 mentions) Qubit fluorometer, by Agilent Technologies (2 mentions)

Close spelling variants of this product

ThruPLEX Kit (7 citations)

2 protocols using thruplex dnaseq sample preparation kits

1

Low-Pass WGS for Copy Number

Check if the same lab product or an alternative is used in the 5 most similar protocols
Copy number characterization was performed using low-pass (~0.2x coverage) whole-genome sequencing. Libraries were prepared from 50ng of DNA using ThruPLEX-DNAseq sample preparation kits (Rubicon Genomics), according to the manufacturer’s protocol. The resultant libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Library Quantification kit (Kapa Biosystems), according to the manufacturer’s protocol. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on a single Illumina NextSeq500 run with paired-end 35bp reads, at the Dana-Farber Cancer Institute Molecular Biology Core Facilities. The reads were aligned to the UCSC hg19 reference genome, using “BWA-MEM” (v0.07.15), with default parameters.
Ben-David U., Siranosian B., Ha G., Tang H., Oren Y., Hinohara K., Strathdee C.A., Dempster J., Lyons N.J., Burns R., Nag A., Kugener G., Cimini B., Tsvetkov P., Maruvka Y.E., O’Rourke R., Garrity A., Tubelli A.A., Bandopadhayay P., Tsherniak A., Vazquez F., Wong B., Birger C., Ghandi M., Thorner A.R., Bittker J.A., Meyerson M., Getz G., Beroukhim R, & Golub T.R. (2018). Genetic and transcriptional evolution alters cancer cell line drug response. Nature, 560(7718), 325-330.
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2

Low-Pass WGS for Copy Number

Check if the same lab product or an alternative is used in the 5 most similar protocols
Copy number characterization was performed using low-pass (~0.2x coverage) whole-genome sequencing. Libraries were prepared from 50ng of DNA using ThruPLEX-DNAseq sample preparation kits (Rubicon Genomics), according to the manufacturer’s protocol. The resultant libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Library Quantification kit (Kapa Biosystems), according to the manufacturer’s protocol. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on a single Illumina NextSeq500 run with paired-end 35bp reads, at the Dana-Farber Cancer Institute Molecular Biology Core Facilities. The reads were aligned to the UCSC hg19 reference genome, using “BWA-MEM” (v0.07.15), with default parameters.
Ben-David U., Siranosian B., Ha G., Tang H., Oren Y., Hinohara K., Strathdee C.A., Dempster J., Lyons N.J., Burns R., Nag A., Kugener G., Cimini B., Tsvetkov P., Maruvka Y.E., O’Rourke R., Garrity A., Tubelli A.A., Bandopadhayay P., Tsherniak A., Vazquez F., Wong B., Birger C., Ghandi M., Thorner A.R., Bittker J.A., Meyerson M., Getz G., Beroukhim R, & Golub T.R. (2018). Genetic and transcriptional evolution alters cancer cell line drug response. Nature, 560(7718), 325-330.
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