Su8230 scanning electron microscope

Manufactured by Hitachi

Sourced in Japan, United Kingdom

The SU8230 is a high-performance scanning electron microscope (SEM) manufactured by Hitachi. It is designed to provide high-resolution imaging and detailed analysis of a wide range of sample types. The SU8230 features a field emission electron gun that delivers a high-quality electron beam, enabling the microscope to capture detailed images at high magnification. It is equipped with advanced imaging and analytical capabilities to support a variety of applications in materials science, nanotechnology, and other research fields.

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Lab products found in correlation

Axioscope 5, by Zeiss (1 mentions) X max 1160 edx, by Oxford Instruments (1 mentions) Ft ir 610 spectrometer, by Jasco (1 mentions) Bx61 microscope, by Olympus (1 mentions) V 570 spectrophotometer, by Jasco (1 mentions) D8 advance diffractometer, by Bruker (1 mentions) Smartsem software, by Zeiss (1 mentions)

11 protocols using su8230 scanning electron microscope

Fibrin Fiber Thickness in Type 1 Diabetes

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To assess fibrin fiber thickness in T1DM and controls, clots were formed in duplicate in the pierced lids of 0.6 mL centrifuge tubes in 45 µL volumes of pooled plasma diluted onefold in buffer (50 mM Tris, 100 mM NaCl, pH 7.4). The plasma samples were pooled from six randomly chosen subjects from each of the T1DM and control group. Clotting was induced by addition of 5 µL of 25 mM CaCl
₂and 5 U/mL thrombin in buffer (50 mM Tris, 100 mM NaCl, pH 7.4). The clots were incubated for 2 hours at 100% humidity. For fixation, clots were given three washes (for 40 minutes each) with 67 mM sodium cacodylate, pH 7.4 and an overnight wash in 2% glutaraldehyde in sodium cacodylate buffer. Samples were dehydrated using a series of acetone washes and dried in a critical point drier. The clots were mounted on SEM stubs, coated with a 4 nm layer of iridium and viewed and photographed at ×10,000 magnification using a SU8230 scanning electron microscope (Hitachi; Maidenhead, UK) operating at 10 kV. Five images per sample were acquired and they were analyzed with Adobe Photoshop (Adobe Systems; San Jose, CA, United States); the diameters of 50 fibers per picture were measured. The images were adjusted for brightness and contrast and cropped.

Sobczak A.I., Phoenix F.A., Pitt S.J., Ajjan R.A, & Stewart A.J. (2020). Reduced Plasma Magnesium Levels in Type-1 Diabetes Associate with Prothrombotic Changes in Fibrin Clotting and Fibrinolysis. Thrombosis and Haemostasis, 120(2), 243-252.

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Microstructural Characterization by SEM

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Scanning electron microscopy images were obtained using the equipment present within our group, namely a Hitachi SU 8230 Scanning Electron Microscope (Tokyo, Japan). Microstructural characterization was performed using the scanning electron microscope and all the lyophilized samples were analyzed in section without being coated with a conductive layer, taking advantage of low voltages imaging capabilities and/or deceleration voltages of the e-beam.

Marin Ș., Albu Kaya M.G., Ghica M.V., Dinu-Pîrvu C., Popa L., Udeanu D.I., Mihai G, & Enachescu M. (2018). Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings. Pharmaceutics, 10(4), 224.

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Morphological Analysis of Fe3O4, Fe3O4-Au, and Au-Ag

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The morphology and the configuration of the Fe₃O₄, Fe₃O₄-Au, and Au-Ag samples were determined by high-resolution scanning electron microscopy (HRSEM) through a Hitachi SU-8230 Scanning Electron Microscope (2535 Augustine Drive, Santa Clara, CA 95054, USA). The microscope was equipped with a cold field emission device.

Ruíz-Baltazar Á.D., Böhnel H.N., Larrañaga Ordaz D., Cervantes-Chávez J.A., Méndez-Lozano N, & Reyes-López S.Y. (2023). Green Ultrasound-Assisted Synthesis of Surface-Decorated Nanoparticles of Fe3O4 with Au and Ag: Study of the Antifungal and Antibacterial Activity. Journal of Functional Biomaterials, 14(6), 304.

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Multitechnique Characterization of Samples

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Optical microscopy investigation was effectuated using a transmitted light biological microscope (ZEISS Axioscope 5, Oberocken, Germany).
Scanning Electron Microscopy was effectuated using a Hitachi SU8230 Scanning Electron Microscope, Tokyo, Japan, at an acceleration voltage of 30 kV in low-vacuum mode without sample metallization. Elemental analysis was effectuated with an Energy-Dispersive Spectroscopy (EDS) detector: X-Max 1160 EDX (Oxford Instruments, Oxford, UK). The culture medium within wells was removed before SEM investigation and afterwards rinsed with ultrapure water and stored under a desiccator for 1 h until their surface was completely dry.
Fourier-transform infrared spectroscopy (FTIR) was performed using an FTIR 610 spectrometer (Jasco Corporation, Tokyo, Japan) in the wavenumber range of 4000–400 cm⁻¹, using the potassium bromide pellet technique. Each spectrum was registered at a resolution of 4 cm⁻¹ and represents the average of 100 scans.

Ardelean A.I., Dragomir M.F., Moldovan M., Sarosi C., Paltinean G.A., Pall E., Tudoran L.B., Petean I, & Oana L. (2023). In Vitro Study of Composite Cements on Mesenchymal Stem Cells of Palatal Origin. International Journal of Molecular Sciences, 24(13), 10911.

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Quantifying Zn and Zr via EDX-SEM

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Energy-dispersive X-ray
spectroscopy was performed using a Hitachi SU8230 scanning electron
microscope (SEM) operated at a 20 kV acceleration voltage, a 1 k magnification,
and a 30 μA current. Both Zn and Zr were quantified at their
respective L-edges (see the example in Figure S2).

Redekop E.A., Cordero-Lanzac T., Salusso D., Pokle A., Oien-Odegaard S., Sunding M.F., Diplas S., Negri C., Borfecchia E., Bordiga S, & Olsbye U. (2023). Zn Redistribution and Volatility in ZnZrOx Catalysts for CO2 Hydrogenation. Chemistry of Materials, 35(24), 10434-10445.

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Elemental Composition Determination by EDS

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The chemical composition of the samples was determined by energy-dispersive X-ray spectroscopy (EDS). The elemental compositions of the samples were collected with a Bruker X Flash 6/60 system coupled to the Hitachi SU-8230 Scanning Electron Microscope.

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Fibrin Fiber Thickness in T1DM

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To assess fibrin fiber thickness in T1DM and controls, clots were formed in duplicate in the pierced lids of 0.6 mL centrifuge tubes in 45 μL volumes of pooled plasma diluted onefold in buffer (50 mM Tris, 100 mM NaCl, pH 7.4). The plasma samples were pooled from six randomly chosen subjects from each of the T1DM and control group. Clotting was induced by addition of 5 μL of 25 mM CaCl₂ and 5 U/mL thrombin in buffer (50 mM Tris, 100 mM NaCl, pH 7.4). The clots were incubated for 2 hours at 100% humidity. For fixation, clots were given three washes (for 40 minutes each) with 67 mM sodium cacodylate, pH 7.4 and an overnight wash in 2% glutaraldehyde in sodium cacodylate buffer. Samples were dehydrated using a series of acetone washes and dried in a critical point drier. The clots were mounted on SEM stubs, coated with a 4 nm layer of iridium and viewed and photographed at ×10,000 magnification using a SU8230 scanning electron microscope (Hitachi; Maidenhead, UK) operating at 10 kV. Five images per sample were acquired and they were analyzed with Adobe Photoshop (Adobe Systems; San Jose, CA, United States); the diameters of 50 fibers per picture were measured. The images were adjusted for brightness and contrast and cropped.

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Microscopic Analysis of Adult Parasites

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All adult worms were washed in saline, preserved for 24 h in 0.5 % formalin, dehydrated, cleared in lactophenol, mounted in Canada balsam and analyzed by light microscopy using an Olympus BX 61 microscope (Japan). For scanning electron microscopy, some adult parasites were fixed for 2 h at 4 °C in 2.7 % glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) and washed in PBS. Samples were post-fixed for 1 h with 1 % OsO₄. The parasites were dehydrated in an ethanol series (30–100 %), and infiltrated with hexamethyldisilazane, dried, mounted on aluminum stubs coated with a 10 nm gold layer, and examined with a Hitachi SU8230 Scanning Electron Microscope (Japan).

Gherman C.M., Deak G., Matei I.A., Ionică A.M., D’Amico G., Taulescu M., Barbu-Tudoran L., Sarmaşi A., Mihalca A.D, & Cozma V. (2016). A rare cardiopulmonary parasite of the European badger, Meles meles: first description of the larvae, ultrastructure, pathological changes and molecular identification of Angiostrongylus daskalovi Janchev & Genov 1988. Parasites & Vectors, 9, 423.

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Comprehensive Characterization of Cotton and Leather

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Hitachi SU-8230 scanning electron microscope (Tokyo, Japan) operated at 30 kV was used to observe the morphology and elemental composition of final cotton and leather samples. The crystalline phases were identified by X-ray powder diffraction (XRD) on Bruker D8 Advance diffractometer (Karlsruhe, Germany) using CuK_α1 radiation (λ = 1.540598 Å). The diffraction peaks were identified from ICDD’s Powder Diffraction File (PDF) database with Match! version 1.11k software. Attenuated total reflection–Fourier transform infrared spectroscopy (ATR-FTIR) was recorded on FT-IR Jasco 6100 spectrometer (Jascp International Co, Ltd., Tokyo, Japan) to determine the chemical structure of the cotton and leather samples. Investigation of the optical absorption properties of all samples was carried out using a Jasco V-570 Spectrophotometer (Jasco International Co., Ltd., Tokyo, Japan).

Cosma D.V., Tudoran C., Coroș M., Socaci C., Urda A., Turza A., Roșu M.C., Barbu-Tudoran L, & Stanculescu I. (2023). Modification of Cotton and Leather Surfaces Using Cold Atmospheric Pressure Plasma and TiO2-SiO2-Reduced Graphene Oxide Nanopowders. Materials, 16(4), 1397.

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Zinc-Induced Fibrin Clot Ultrastructure

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Clots were formed in duplicate from either: (1) 10 μM purified fibrinogen and 300 μM HSA in buffer in the presence and absence of 40 μM added Zn²⁺. (2) Pooled-plasma from 6 randomly chosen patients with T2DM and pooled-plasma from 6 age-matched controls in the presence and absence of 116 μM added Zn²⁺. Clots were prepared by diluting 1 : 1 in buffer either the purified system or the plasma and subsequently 45 μL of these diluted solutions were added to 5 μL of activation mix containing 2.5 U mL⁻¹ thrombin and 25 mM CaCl₂ in buffer. The clots were then processed by stepwise dehydration as previously described.^{37 (link)} All clots were viewed and photographed at ×5000, ×10 000, ×25 000 and ×30 000 magnification using a SU8230 scanning electron microscope (Hitachi, Maidenhead, UK) in 5 different areas. The diameter of 50 fibres in each image was measured using Adobe Photoshop (Adobe Systems, San Jose, CA). The mean diameter of the fibres in each image was used to compare each sample type.

Sobczak A.I., Katundu K.G., Phoenix F.A., Khazaipoul S., Yu R., Lampiao F., Stefanowicz F., Blindauer C.A., Pitt S.J., Smith T.K., Ajjan R.A, & Stewart A.J. (2021). Albumin-mediated alteration of plasma zinc speciation by fatty acids modulates blood clotting in type-2 diabetes. Chemical Science, 12(11), 4079-4093.

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