Aldoa 11217 1 ap

Manufactured by Proteintech

ALDOA (11217-1-AP) is a primary antibody that specifically recognizes the Aldolase A protein. Aldolase A is an enzyme involved in glycolysis, the metabolic pathway that converts glucose into pyruvate.

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Lab products found in correlation

Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Hk2 22029 1 ap, by Proteintech (1 mentions)

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ALDOA (3 citations)

3 protocols using aldoa 11217 1 ap

Protein Expression Analysis in Cervical Cancer

Cited 1 time

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The expression levels of various proteins in Siha and Caski cells were determined by Western blot analysis as previously described.26 (link) Antibodies to GLUT1(21829-1-AP), LDHA (19987-1-AP), and ALDOA (11217-1-AP) were acquired from Proteintech TM. Antibodies to ITGB7, FAK, p-FAK (Tyr 925), AKT1, p-AKT1 (Ser 473), C/EBPβ, and p-C/EBPβ (Thr 235) were from Abcam. The antibody to β-actin (A5441) was obtained from Sigma-Aldrich.

Chai Z., Yang Y., Gu Z., Cai X., Ye W., Kong L., Qiu X., Ying L., Wang Z, & Wang L. (2019). Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPβ Signaling Pathway in Cervical Cancer Cell Lines. OncoTargets and therapy, 12, 10415-10425.

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Western Blot Analysis of ADAM17 and ALDOA

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Protein solution was diluted by sample buffer (4×) containing reducing reagent and heated at 95°C for 5 minutes. Proteins were separated on 4%-12% gradient sodium dodecyl sulphate-polyacrylamide gel and then transferred to a PVDF membrane (Invitrogen, CA). The membranes were blocked for 1 h in PBS containing 5% nonfat dry milk (v/v), and then incubated for 1 h with the primary antibody at room temperature. After 4 washes with 0.1% (v/v) Tween 20 in PBS (PBS-T), the membranes were incubated for 1 h with the appropriate secondary antibody. Finally, the membranes were washed 4 times with PBS-T, and the bound antibodies were visualized with the Pierce ECL Western Blotting Substrate (ThermoFisher Scientific, IL). Beta actin was used as control (Sigma A3854). ADAM17 (24620-1-AP) and ALDOA (11217-1-AP) were both purchased from proteintech (Rosemont, IL).

Sendi H., Yazdimamaghani M., Hu M., Sultanpuram N., Wang J., Moody A.S., McCabe E., Zhang J., Graboski A., Li L., Rojas J.D., Dayton P.A., Huang L, & Wang A.Z. (2021). Nanoparticle delivery of miR-122 inhibits colorectal cancer liver metastasis. Cancer research, 82(1), 105-113.

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Western Blot Analysis of EMT Markers

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The specific primary antibodies against E-cadherin (ET1607–75), N-cadherin (ET1607–37), snail (ER1706–22), Flag (M1403–2), p-AKT(ET1607–73), AKT (ET1609–51), p-ERK (ET1610–13), ERK (ET1601–29), p-MEK (ET1609–50), CXCR4 (ER1802–28) and ENO1 (ET1705–56) were ordered from Hangzhou Hua-An Biotechnology Company. Other antibodies included β-actin (TA-09, ZSGB-BIO), MEK (sc-436, Santa Cruz), HK2 (22029–1-AP, Proteintech), ALDOA (11217–1-AP, Proteintech) and PGK1 antibody (sc-130335, Santa Cruz).
For serum PGK1 detection, the high abundance of serum albumin and IgG was deleted [21 (link), 22 (link)] using a reagent kit following procedures (Albumin and IgG Erasin Kit, C500063–0005, Shanghai Sangon Biotechnology). Reversible Ponceau staining was used as a loading control.
The density of target band in Western blot was measured with Image J software (NIH, USA) for semi-quantification of staining signals.

He Y., Wang X., Lu W., Zhang D., Huang L., Luo Y., Xiong L., Li H., Zhang P., Li Q, & Liang S. (2022). PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis. Cell Death & Disease, 13(2), 118.

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