Exocettm assay

Manufactured by System Biosciences

The EXOCETTM assay is a kit designed to quantify the concentration of extracellular vesicles (EVs) in biological samples. The assay utilizes a fluorescent lipid dye to label the lipid bilayer of EVs, allowing for their detection and quantification using a fluorescence-based readout.

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Lab products found in correlation

Exoquick exoq5tm 1, by System Biosciences (1 mentions) Bca assay kit, by Thermo Fisher Scientific (1 mentions)

4 protocols using exocettm assay

Exosome Quantification by Assays

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Exosome concentration was estimated using the EXOCET^TM assay (System Biosciences), performed as described previously.48 (link) Quantification of exosomes by fluorescence polarization (FP) was performed as described previously.49 (link)

Gurunathan S., Kang M.H., Jeyaraj M, & Kim J.H. (2021). Platinum Nanoparticles Enhance Exosome Release in Human Lung Epithelial Adenocarcinoma Cancer Cells (A549): Oxidative Stress and the Ceramide Pathway are Key Players. International Journal of Nanomedicine, 16, 515-538.

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Exosome Quantification using EXOCET Assay

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Exosome concentration was estimated using the EXOCET^TM assay (System Biosciences), performed as described previously.48 (link)

Gurunathan S., Kang M.H., Jeyaraj M, & Kim J.H. (2021). Palladium Nanoparticle-Induced Oxidative Stress, Endoplasmic Reticulum Stress, Apoptosis, and Immunomodulation Enhance the Biogenesis and Release of Exosome in Human Leukemia Monocytic Cells (THP-1). International Journal of Nanomedicine, 16, 2849-2877.

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Exosome Quantification via EXOCET Assay

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In addition to NTA, exosome concentration was also estimated using the EXOCET^TM assay (System Biosciences). This method is based on the measurement of the Acetyl-CoA Acetylcholinesterase enriched activity in exosomes (37). For each preparation condition, 10 μl of exosomal preparation in PBS were diluted in 90 μl of lysis buffer. This dilution was carried out because for serum and plasma, exosomal preparations’ final values were above the standard curve. The mixture was then incubated at 37°C for 5 min, vortexed and centrifuged at 1500 g at RT during 5 min. The subsequent steps of exosome particle quantitation were performed according to manufacturer’s instructions and absorbance was measured at 405 nm using the Infinite M200 plate reader (TECAN^TM).

Soares Martins T., Catita J., Martins Rosa I., A. B. da Cruz e Silva O, & Henriques A.G. (2018). Exosome isolation from distinct biofluids using precipitation and column-based approaches. PLoS ONE, 13(6), e0198820.

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Exosome Isolation and Characterization Protocol

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Exosomes were isolated and purified using ExoQuick (EXOQ5TM-1, System Biosciences, Palo Alto, CA, USA) following the manufacturer’s instructions. SEM analysis was performed according to a previously described method.60 (link) Sample preparation for TEM and analysis were performed according to a previously described method.60 (link) The total protein concentration of exosomes was determined using the bicinchoninic acid (BCA) assay kit (Thermo Scientific, Waltham, MA, USA) following the manufacturer’s instructions. Exosome concentration was estimated using the EXOCET^TM assay (System Biosciences), performed as described previously.28 (link) Quantification of exosomes by fluorescence polarization (FP) was performed as described previously.61 (link)

Gurunathan S, & Kim J.H. (2022). Graphene Oxide Enhances Biogenesis and Release of Exosomes in Human Ovarian Cancer Cells. International Journal of Nanomedicine, 17, 5697-5731.

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