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Magnetic carboxylated beads

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Magnetic carboxylated beads are spherical particles with a magnetic core and a carboxylated surface. They are designed for use in various research and diagnostic applications that require magnetic separation or capture of target analytes.

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Lab products found in correlation

Tetanus toxoid, by Merck Group (3 mentions) Influenza hemagglutinin h1cal2009, by Sino Biological (3 mentions) Siv gp120, by Sino Biological (3 mentions) Spike 2, by ACROBiosystems (2 mentions) Sars cov 2 spike 1, by Sino Biological (2 mentions) Sars cov 2 and hku 1 spike trimers, by BPS Biosciences (2 mentions) Sars cov and nl63 spike trimers, by BPS Biosciences (2 mentions) Esat 6, by BEI Resources (1 mentions) Nucleoprotein, by ACROBiosystems (1 mentions) Sars cov 2 spike trimers, by BPS Biosciences (1 mentions)

Close spelling variants of this product

Carboxylated beads (4 citations) Magnetic beads (43 citations)

4 protocols using magnetic carboxylated beads

1

Multiplex SARS-CoV-2 Antibody Detection

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As previously described25 , a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Fig. 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were also included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B., Selva K., Kedzierski L., Ashhurst T., Haycroft E., Shoffner S., Hensen L., Boyd D., James F., Mouhtouris E., Kwong J., Chua K., Drewett G., Copaescu A., Dobson J., Rowntree L., Habel J., Allen L., Koay H.F., Neil J., Gartner M., Lee C., Andersson P., Seemann T., Sherry N., Amanat F., Krammer F., Londrigan S., Wakim L., King N., Godfrey D., Mackay L., Thomas P., Nicholson S., Arnold K., Chung A., Holmes N., Smibert O., Trubiano J., Gordon C., Nguyen T, & Kedzierska K. (2021). Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity. Research Square.
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2

Multiplex Assay for Tuberculosis Antibodies

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A customised TB multiplex assay was designed to assess for Ab specific for Ag85B, MPT64, TX114 proteins, TB peptidoglycan, Ag85 complex and ESAT‐6 (BEI resources, Manassas, USA). ATBI‐positive serum from a different cohort was used as a control to verify Mtb antigen binding. H3 influenza haemagglutinin (Sino Biological A/Switzerland/9715293/2013, HA; Sino Biological, Beijing, China) was also included in the array as a positive antigen control. Briefly, magnetic carboxylated beads (Bio‐Rad, California, USA) were covalently coupled to Mtb and Flu antigens by carbodiimide reaction as previously described.47 The isotypes and subclasses (IgG, IgA1, IgA2, IgG1‐4) of antigen‐specific Abs were assessed as previously described.48
McLean M.R., Wragg K.M., Lopez E., Kiazyk S.A., Ball T.B., Bueti J., Kent S.J., Juno J.A, & Chung A.W. (2021). Serological and cellular inflammatory signatures in end‐stage kidney disease and latent tuberculosis. Clinical & Translational Immunology, 10(11), e1355.
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3

SARS-CoV-2 Multiplex Serological Assay

Cited 1 time
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As previously described25 (link), a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Table 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B.Y., Selva K.J., Kedzierski L., Ashhurst T.M., Haycroft E.R., Shoffner-Beck S.K., Hensen L., Boyd D.F., James F., Mouhtouris E., Kwong J.C., Chua K.Y., Drewett G., Copaescu A., Dobson J.E., Rowntree L.C., Habel J.R., Allen L.F., Koay H.F., Neil J.A., Gartner M.J., Lee C.Y., Andersson P., Khan S.F., Blakeway L., Wisniewski J., McMahon J.H., Vine E.E., Cunningham A.L., Audsley J., Thevarajan I., Seemann T., Sherry N.L., Amanat F., Krammer F., Londrigan S.L., Wakim L.M., King N.J., Godfrey D.I., Mackay L.K., Thomas P.G., Nicholson S., Arnold K.B., Chung A.W., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Nguyen T.H, & Kedzierska K. (2022). SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity. Nature Communications, 13, 2774.
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4

Multiplex Bead Array for COVID-19 Serology

Cited 1 time
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A custom multiplex bead array was designed (Supplemental Table 3) and coupled with SARS-CoV-1 and SARS-CoV-2 spike-1 (stem, Sino Biological), spike-2 (head, ACRO Biosystems), RBD (BEI Resources), and N (ACRO Biosystems), as described previously (45 (link)). In addition, SARS-CoV-2 spike trimers (provided in-house) and SARS-CoV-2 spike trimers (BPS Bioscience) were included. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009, Sino Biological), and SIV-gp120 (Sino Biological) were included as positive and negative control antigens, respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio-Rad) using a 2-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS with 0.05% sodium azide until use.
Habel J.R., Chua B.Y., Kedzierski L., Selva K.J., Damelang T., Haycroft E.R., Nguyen T.H., Koay H.F., Nicholson S., McQuilten H.A., Jia X., Allen L.F., Hensen L., Zhang W., van de Sandt C.E., Neil J.A., Pragastis K., Lau J.S., Jumarang J., Allen E.K., Amanant F., Krammer F., Wragg K.M., Juno J.A., Wheatley A.K., Tan H.X., Pell G., Walker S., Audsley J., Reynaldi A., Thevarajan I., Denholm J.T., Subbarao K., Davenport M.P., Hogarth P.M., Godfrey D.I., Cheng A.C., Tong S.Y., Bond K., Williamson D.A., McMahon J.H., Thomas P.G., Pannaraj P.S., James F., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Chung A.W., Whitehead C.L., Kent S.J., Lappas M., Rowntree L.C, & Kedzierska K. (2023). Immune profiling of SARS-CoV-2 infection during pregnancy reveals NK cell and γδ T cell perturbations. JCI Insight, 8(7), e167157.
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