Potassium chloride (kcl)

Manufactured by Euromedex

Sourced in France

Potassium chloride (KCl) is a chemical compound that is commonly used in laboratory settings. It is a white, crystalline salt that is soluble in water. KCl serves as a source of potassium ions, which are essential for various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Sodium chloride (nacl), by Avantor (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Annexinv binding buffer, by BD (1 mentions) Triton x 100, by Euromedex (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Merck Group (1 mentions) Annexin 5 fitc, by BD (1 mentions) Aminocaproic acid, by Merck Group (1 mentions) Glycerol, by Carl Roth (1 mentions) Ethylene diamine tetraacetic acid (edta), by Euromedex (1 mentions) Benzamidine, by Merck Group (1 mentions)

2 protocols using potassium chloride (kcl)

Reagents for Cytotoxicity and Apoptosis Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

YTX (purity ≥ 99%) dissolved in methanol was obtained from Cifga Laboratorio (Lugo, Spain). 4-Nitrophenyl N-acetyl-β-D-glucosaminide (pNAG), propidium iodide solution, MgCl₂, glucose, DNP-human serum albumin (DNP-HSA) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) were from Sigma-Aldrich (Germany). Annexin V-FITC and 10X AnnexinV Binding Buffer were from BD Pharmingen (France). HEPES was from Gibco (Life Technologies). NaCl was from VWR (Belgium). KCl and Triton X-100 were from Euromedex (France). CaCl₂ was from Prolano (Paris, France). Bovine serum albumin (BSA) was from PAA (Austria). Mouse monoclonal anti-DNP IgE has been described previously [21 (link)–23 (link)].

Tobío A., Alfonso A., Madera-Salcedo I., Botana L.M, & Blank U. (2016). Yessotoxin, a Marine Toxin, Exhibits Anti-Allergic and Anti-Tumoural Activities Inhibiting Melanoma Tumour Growth in a Preclinical Model. PLoS ONE, 11(12), e0167572.

+ Open protocol

+ Expand

Cell Lysis by Sonication and Soluble Extract Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lysis was done by sonication. Samples were resuspended in 3 mL of lysis buffer containing 50 mM Hepes-KOH (Euromedex), 200 mM KCl (Euromedex), 1 mM EDTA (Euromedex), 10% glycerol (Roth), 1 mM benzamidine (Sigma), 5 mM aminocaproic acid (Sigma), 1 mM NAD + (Sigma). To precipitate DNA, 0.2 % of sulfate streptomycin was added and samples were centrifuged for 25 min at 13,000 x g at 4°C. The supernatant sample, corresponding to soluble extract, was frozen in liquid nitrogen and conserved at -80°C.

Goussé M., Dell'Aglio E., Curien G., Borland S., Renoud S., Ranquet C, & Chandor-Proust A. (2022). E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins. Protein expression and purification, 195-196.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!