TEM images were obtained with an Inspect S50 (FEI, Hillsboro, Oregon, OH, USA) scanning electron microscope. The analysis was performed with 20 kV as High Voltage. Extinction spectra of colloids were recorded on a Shimadzu 3600 spectrometer (Shimadzu Corp., Kyoto, Japan) equipped with a PMT for light detection in the UV–visible range and an InGaAs detector for the NIR was employed to obtain the plasmon extinction spectra. Samples were placed in quartz cells of 1 cm optical path, after dilution to 30% in MilliQ water (v/v).
3600 spectrometer
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu 3600 spectrometer is a versatile instrument designed for the analysis of various samples. It is capable of performing UV-Vis, NIR, and fluorescence spectroscopy measurements. The 3600 spectrometer features a high-performance optical system and advanced data processing capabilities.
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7 protocols using 3600 spectrometer
1
Characterization of Colloidal Nanoparticles
2
Detailed Characterization of Organic Compound
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Starting material used for synthesis and solvents for the analytical use were bought from commercial sources and were used as such without any further purification. The molecular weight of the compound was obtained using JEOL GCMATE mass spectrometer.1H and 13C NMR was obtained using Bruker Ascend III 400 MHz spectrometer using CDCl3, UV spectra were recorded using Shimadzu 3600 spectrometer with a quartz cell (path length = 1 cm) at room temperature. Emission spectra were recorded using Hitachi High Technologies spectrometer. Single crystal X-ray diffraction was carried out with Bruker Kappa APEXII.
3
Optical Characterization of Nanoparticles
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The capacity of a sample
to absorb and disperse light is measured using UV–visible (UV–vis)
spectroscopy. A light source and a focus lens were placed in front
of the integrated TiO2 NP solution, and the amount of light
absorbed at wavelengths between 200 and 800 nm was calculated using
a Shimadzu 3600 spectrometer. Due to the correlation between the absorption
bands and the diameter aspect ratio of metal nanoparticles, UV–vis
spectroscopy is the most frequently used method confirm the formation
of nanoparticles as well as to characterize their optical characteristics
and electronic structure.
to absorb and disperse light is measured using UV–visible (UV–vis)
spectroscopy. A light source and a focus lens were placed in front
of the integrated TiO2 NP solution, and the amount of light
absorbed at wavelengths between 200 and 800 nm was calculated using
a Shimadzu 3600 spectrometer. Due to the correlation between the absorption
bands and the diameter aspect ratio of metal nanoparticles, UV–vis
spectroscopy is the most frequently used method confirm the formation
of nanoparticles as well as to characterize their optical characteristics
and electronic structure.
4
Characterization of Nanostructured Materials
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The chemical compositions of the samples were characterized by X-ray photoelectron spectroscopy (XPS, Thermo Scientific ESCALAB 250Xi A1440 system, Thermo Fisher Scientific, Waltham, MA, USA). Transmission electron microscopy (TEM) images were obtained using a Hitachi H-800 (JEOL 2100, JEOL Ltd., Tokyo, Japan) transmission electron microscopy at an acceleration voltage of 200 kV. The UV-Vis absorption spectra and catalytic properties were monitored using a SHIMADZU 3600 spectrometer (Shimadzu Corporation, Tokyo, Japan).
5
UV–vis absorption of AgNS and AgNPs
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UV–vis absorption spectra of AgNS and AgNPs were recorded from on a Shimadzu 3600 spectrometer by using quartz cells, 1 cm optical path. Samples were diluted 30% in MilliQ water (volume/volume). The UV–visible spectrum of AgNS shows two maxima at 384 and 418 nm, while a large extinction with a broad tail towards the red-near infrared is seen at higher wavelength which is due to the presence of AgNS with different morphologies.
6
Synthesis and Characterization of Zn1-xNdxO
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Zn1 − xNdxO was synthesized using the coprecipitation method. In brief, Zn(NO3)2·6H2O and Nd(NO3)3·6H2O were dissolved in deionized water with a molar ratio where Nd/(Nd + Zn) was x:1 (x = 0.00, 0.005, 0.01, 0.015, 0.0175, 0.02, 0.0225, 0.03). After stirring for 20 min, NH4HCO3 aqueous solution was added. After stirring for 4 h, the white precipitates were collected by centrifugation, washed with deionized water and ethanol several times, and then dried under vacuum at 80 °C for 12 h. Finally, the sample was further annealed in air for 1 h at 600 °C to obtain the final Zn1 − xNdxO (x = 0.00, 0.005, 0.01, 0.015, 0.0175, 0.02, 0.0225, and 0.03) products.
We evaluated the structural quality of the samples with X-ray diffraction (XRD, Rigaku D/Max 3C). X-ray photoelectron spectroscopy (XPS, VG ESCALAB 250X) was used to analyze the element content of the samples. The morphology was characterized by field emission SEM (JEOL JSM-6700F) and TEM (JEM-2100HR). UV–vis absorption spectra were measured by a Shimadzu 3600 spectrometer. Under a 514.5 nm (2.41 eV) Ar+ ion laser, the Renishaw inVia Raman system detected all SERS and PL signals with a laser power of 40 mW, attenuation of 100%, 10-s exposure time, and 1 scan.
We evaluated the structural quality of the samples with X-ray diffraction (XRD, Rigaku D/Max 3C). X-ray photoelectron spectroscopy (XPS, VG ESCALAB 250X) was used to analyze the element content of the samples. The morphology was characterized by field emission SEM (JEOL JSM-6700F) and TEM (JEM-2100HR). UV–vis absorption spectra were measured by a Shimadzu 3600 spectrometer. Under a 514.5 nm (2.41 eV) Ar+ ion laser, the Renishaw inVia Raman system detected all SERS and PL signals with a laser power of 40 mW, attenuation of 100%, 10-s exposure time, and 1 scan.
7
Antimicrobial-Loaded Monofilament Sutures
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Poly(GL)-b-poly(GL-co-TMC-co-CL)-b-poly(GL) monofilaments (5 cm length) were immersed (during 5 s) in ethanol or methanol solutions containing different percentages of CHX (0.1-15 w/v-%) or PHMB (0.1-6 w/v-%), respectively. After drying in hot air sutures were immersed in an ethyl acetate bath containing 3 w/v-% of poly(LA-co-TMC) when coated samples were required. Monofilaments were finally dried and stored under vacuum.
The total amount of drug loaded was determined by dissolution of the suture and the drug in 1,1,1,3,3,3-hexafluoroisopropanol, precipitation of polymers by addition of ethanol and finally by absorbance measurements by UV spectroscopy of the resulting solution using a Shimadzu 3600 spectrometer. Calibration curves were obtained by plotting the absorbance measured at 261 and 236 nm versus CHX and PHMB concentrations, respectively.
The total amount of drug loaded was determined by dissolution of the suture and the drug in 1,1,1,3,3,3-hexafluoroisopropanol, precipitation of polymers by addition of ethanol and finally by absorbance measurements by UV spectroscopy of the resulting solution using a Shimadzu 3600 spectrometer. Calibration curves were obtained by plotting the absorbance measured at 261 and 236 nm versus CHX and PHMB concentrations, respectively.
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