Tcs sp8 multiphoton mp

Manufactured by Leica

The Leica TCS SP8 multiphoton (MP) is a high-performance confocal microscope system designed for advanced imaging applications. It combines the power of multiphoton excitation with the precision of confocal scanning to enable deep tissue imaging and live-cell analysis.

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TCS SP8 MP multiphoton imaging system TCS SP8 MP multiphoton microscope TCS-SP8 multiphoton TCS SP8 MP SP8 multiphoton SP8-MP TCS SP8

2 protocols using tcs sp8 multiphoton mp

Fluorescence Microscopy of Spermathecae

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Spermathecae and RTs were imaged in a Leica TCS SP8 multiphoton (MP) laser scanning confocal microscope operated by the LAS X software. Fluorescence was detected by using argon excitation lasers of 488 nm (for GFP and YFP), which signal was captured by a Hybrid detector (HyD), 552 nm laser (for nRFP) captured by a conventional photomultiplier (PMT) and 638 nm (for LyTR) by a second HyD. The size and number of optical sections from different focal planes were collected according to the following experimental sets.

Sanchez-Lopez J.A., Twena S., Apel I., Kornhaeuser S.C., Chasnitsky M., Miklosi A.G., Vega-Dominguez P.J., Shephard A., Hefetz A, & Heifetz Y. (2022). Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila. Communications Biology, 5, 815.

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Multiphoton Imaging of Reproductive Tracts

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Reproductive tracts were imaged in a Leica TCS SP8 multiphoton (MP) laser scanning confocal microscope operated by the LAS X software. Fluorescence was detected by using argon excitation lasers of 488 nm captured by a conventional photomultiplier (PMT). Image processing was done using Fiji and Imaris 8.4 (Bitplane).

McQueen E.W., Afkhami M., Atallah J., Belote J.M., Gompel N., Heifetz Y., Kamimura Y., Kornhauser S.C., Masly J.P., O’Grady P., Peláez J., Rebeiz M., Rice G., Sánchez-Herrero E., Santos Nunes M.D., Santos Rampasso A., Schnakenberg S.L., Siegal M.L., Takahashi A., Tanaka K.M., Turetzek N., Zelinger E., Courtier-Orgogozo V., Toda M.J., Wolfner M.F, & Yassin A. (2022). A standardized nomenclature and atlas of the female terminalia of Drosophila melanogaster. Fly, 16(1), 128-151.

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