Roswell Park Memorial Institute (RPMI)-1640 medium (LM011-01), fetal bovine serum (FBS; S001-07), and trypsin (LS015-01) were obtained from WELGENE Inc. Phosphate buffered saline (PBS; 17-517Q) was obtained from Lonza. ProLong® Gold antifade reagent (P36931) containing 4,6-diamidino-2-phenylindole (DAPI), and antibodies for claudin-1 (51-9000) and occludin (40-6100) were purchased from Invitrogen. Antibodies for phospho-extracellular signal-regulated kinases1/2 (p-ERK1/2) (9101) and ERK1/2 (9102) were purchased from Cell Signaling Technology. Antibodies for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; sc-25778), p65 (sc-372), Lamin B (sc-6216), goat anti-rabbit IgG-HRP (sc-2030), donkey anti-goat IgG-HRP (sc-2020), and an ERK1/2 inhibitor [PD98059 (PD); sc-3532] were obtained from Santa Cruz Biotechnology, Inc. DyLight™-488-conjugated anti-IgG (A120-10872) was purchased from Bethyl Laboratories. Anhydrous aluminum chloride (563919; Al 20.24%, Cl 79.76%) an nuclear factor-kappa B ( ) inhibitor [Bay11-7085 (Bay); B5681], and Pierce bicinchoninic acid (BCA) protein assay kit (23225) were purchased from Sigma-Aldrich. Nitrocellulose membrane (10600002) was obtained from (GE Healthcare Bio-Sciences).
Fetal bovine serum fbs s001 07
Manufactured by Welgene
Fetal bovine serum (FBS; S001-07) is a common cell culture supplement derived from the blood of bovine fetuses. It provides a complex mixture of proteins, growth factors, and other nutrients that support the growth and proliferation of cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Anhydrous aluminum chloride, by Merck Group (1 mentions)
Erk1 2 9102, by Cell Signaling Technology (1 mentions)
Donkey anti goat igg hrp, by Santa Cruz Biotechnology (1 mentions)
Perk1 2 9101, by Cell Signaling Technology (1 mentions)
Murine raw264.7 macrophages, by American Type Culture Collection (1 mentions)
2 protocols using fetal bovine serum fbs s001 07
1
Analysis of Cell Tight Junction Proteins
2
Murine Macrophage Inflammation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Murine RAW264.7 macrophages were purchased from the American Type Culture Collection (ATCC TIB-71; ATCC, Manassas, VA, USA). Dulbecco’s modified Eagle’s medium (DMEM; low glucose, 1000 mg/L; phenol red, LM 001-11) and fetal bovine serum (FBS; S 001-07) were purchased from Welgene Inc. (Daegu, Korea). LPS (cat. no. L6529), tCIN (cat. no. C80687), vitamin C (cat. no. A0278), 1,1-diphenyl-2-picrylhydrazyl (DPPH; cat. no. D9132), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS; cat. no. A1888) were purchased from Sigma-Aldrich (St. Louis, MO, USA). β-CD (cat. no. 030-08342) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The nitric oxide (NO) detection kit (cat. no. ADI-917-010) was purchased from Enzo Life Sciences (Farmingdale, NY, USA).
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