Cell apoptosis and cell cycle were determined by Flow cytometry. Briefly, cells were cultured in normal medium and transfected with corresponding siRNA or pcDNA, respectively. Cells were collected after transfection for 48 h. Cell apoptosis was determined by PE Annexin V apoptosis detection kits (BD Pharmingen, San Diego, CA, USA). Cell cycle analysis was performed adopting propidium iodide cell cycle detected kits (BD Pharmingen). Finally, cell apoptosis and cell cycle were determined using flow cytometry (EPICS, XL-4, Beckman, CA, USA). Experiments were repeated at least three times.
Propidium iodide cell cycle detected kits
Manufactured by BD
Sourced in United States
Propidium iodide cell cycle detection kits are fluorescent dyes used to stain cellular DNA. They allow for the quantification of cells in different phases of the cell cycle through flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using propidium iodide cell cycle detected kits
1
Apoptosis and Cell Cycle Analysis
2
Cell Cycle and Apoptosis Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze cell cycle progression of transfected LAD cells, propidium iodide cell cycle detected kits (BD, U.S.A.) were applied under the manufacturer’s directions. And cell apoptosis was determined by Annexin V-FITC apoptosis detection kits (Selleck, Shanghai, China) on the basis of the manufacturer’s instructions. The results were evaluated using flow cytometry (FACSCalibur, BD Biosciences). The experiments were carried out in triplicates.
3
Cell Apoptosis and Cell Cycle Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis and cell cycle were evaluated by Flow cytometry. Cells were collected after transfection for 48 h. Cell apoptosis was determined using PE Annexin V apoptosis detection kits (BD Pharmingen, San Diego, CA, USA) following the instructions. Cell cycle analysis was determined using propidium iodide cell cycle detected kits (BD Pharmingen) according to the instructions. Finally, cell apoptosis and cell cycle were determined using flow cytometry.
4
Apoptosis and Cell Cycle Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 48 h transfection, cells were collected and washed with PBS. FACS analysis for apoptosis was done using PE Annexin V apoptosis detection kits (BD Biosciences) after 48 h transfection according to the manufacturer's protocol. Cell cycle analysis was performed adopting propidium iodide cell cycle detected kits (BD Biosciences) according to the manufacturer's protocol. Each experiment was performed in triplicate.
5
Cell Cycle Analysis with Propidium Iodide
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cycle analysis was performed adopting propidium iodide cell cycle detected kits (BD Pharmingen; Franklin Lakes, NJ, USA) according to the manufacturer's protocol. All experiments were performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!