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Incucyte zoom incubator

Manufactured by Sartorius
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Sourced in United States

The IncuCyte ZOOM incubator is a live-cell analysis system that enables continuous, long-term monitoring of cells in a controlled environment. It provides real-time quantitative data on cell proliferation, migration, and morphology without the need for cell labeling or disruption.

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Lab products found in correlation

Imagelock microplate, by Sartorius (1 mentions) Woundmaker, by Sartorius (1 mentions) Incucyte software, by Sartorius (1 mentions)

Spelling variants of this product

IncuCyte ZOOM (678 citations) IncuCyte (373 citations) Incucyte incubator (4 citations) IncuCyte Zoom incubator imaging system (3 citations) IncuCyte Zoom in-incubator imaging system (2 citations) 2-color IncuCyte Zoom in-incubator imaging system (2 citations)

5 protocols using incucyte zoom incubator

1

Wound Healing Assay for MDA-MB-231-4175 Cells

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MDA-MB-231-4175 cells were plated in a 96-well ImageLock Microplate (Essen BioScience, catalog no. 4379) at a density of 3.5 × 104 cells/well in RPMI medium. Twenty-four hours after seeding, the WoundMaker (Essen BioScience, catalog no. 4563) was used to create reproducible scratch wounds in each well. The medium was aspirated after wounding, and the cells were washed and treated with αEGFR-E-P125A or equimolar amounts of the control treatments. The assay plate was incubated in an Incucyte ZOOM incubator (Essen BioScience), and wound width was monitored by imaging at the 10x objective every 4 hours. Wound width was characterized by the average distance between the top and bottom edges of the migrating cell population within the scratch wound (microns) and was computed using Incucyte software.
Sankar A.P., Cho H.M., Shin S.U., Sneh T., Ramakrishnan S., Elledge C., Zhang Y., Das R., Gil-Henn H, & Rosenblatt J.D. (2024). Antibody–Drug Conjugate αEGFR-E-P125A Reduces Triple-negative Breast Cancer Vasculogenic Mimicry, Motility, and Metastasis through Inhibition of EGFR, Integrin, and FAK/STAT3 Signaling. Cancer Research Communications, 4(3), 738-756.
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2

Cell Viability Assay with LC Fibrils

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Cell viability experiments were carried out as described previously [5 (link)]. Briefly, RFP-AC16 cardiomyocytes were plated at a concentration of 2,000 cells/well in a 96-well Corning polystyrene plate and allowed to grow overnight for cell attachment (<20 h) in the IncuCyte ZOOM incubator (5% CO2 at 37°C) (Essen Bioscience, Ann Arbor, MI, USA). Next day, cell culture media was replaced with fresh media, with or without LC fibrils (final concentration 1 μM). The changes in cell growth were analyzed by red counts per well every 4 h until cells become over confluent (> 60 h).
Lin Y., Marin-Argany M., Dick C.J., Redhage K.R., Blancas-Mejia L.M., Bulur P., Butler G.W., Deeds M.C., Madden B.J., Williams A., Wall J.S., Dietz A, & Ramirez-Alvarado M. (2017). Mesenchymal stromal cells protect human cardiomyocytes from amyloid fibril damage. Cytotherapy, 19(12), 1426-1437.
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3

HSV1716-GFP Infection in 3D Spheroids

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HSV1716 infection of spheroids was assessed by GFP expression within spheroids infected with HSV1716-GFP. Collagen was overlaid with cell culture medium with or without 8 × 104 PFU/well of HSV1716-GFP. Spheroids were imaged in the IncuCyte ZOOM incubator (Essen BioScience) at 37°C with 5% CO2 in air using the ×4 microscope objective, with images taken hourly for 70 hr. IncuCyte software (Essen BioScience) was used to create movies and visualize GFP expression.
Cockle J.V., Brüning-Richardson A., Scott K.J., Thompson J., Kottke T., Morrison E., Ismail A., Carcaboso A.M., Rose A., Selby P., Conner J., Picton S., Short S., Vile R., Melcher A, & Ilett E. (2017). Oncolytic Herpes Simplex Virus Inhibits Pediatric Brain Tumor Migration and Invasion. Molecular Therapy Oncolytics, 5, 75-86.
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4

Quantifying AC16 Cell Growth in Transwell

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Cultures were set up using the ClearView membrane transwell with 96 laser etched pores with a diameter of 8 μm within the Incucyte ZOOM incubator (Essen Bioscience, Ann Arbor, MI, USA). Unlike co-culture experiments, MSC and RFP-AC16 were cultured in different chambers in a non-migrating transwell plate that do not allow cell-to-cell contact. Cell culture media alone was added on the lower reservoir plate (or bottom compartment) as control. RFP-AC16 cells, in AC16 cell culture media, were added to the upper chamber at a concentration of 1,000 cells/well and allowed to grow overnight for cell attachment (<20 h) in the Incucyte ZOOM incubator (5% CO2 at 37°C). Cell culture media of the lower compartment was replaced with fresh media containing MSC cells at a final concentration of 400, 800, 1800 and 2000 cells/well (ratio MSC:RFP-AC16 is 0.2:1, 0.4:1, 0.8:1 and 1:1 respectively). At the upper compartments, media was exchanged with fresh media containing LC fibrils (final concentration 1 μM) to the attached RFP-AC16 cells. Each condition was set up in triplicate. Red fluorescent counts per well were measured every 4 h for more than 150 h. Fold growth compared to baseline count at the time of media change was calculated.
Lin Y., Marin-Argany M., Dick C.J., Redhage K.R., Blancas-Mejia L.M., Bulur P., Butler G.W., Deeds M.C., Madden B.J., Williams A., Wall J.S., Dietz A, & Ramirez-Alvarado M. (2017). Mesenchymal stromal cells protect human cardiomyocytes from amyloid fibril damage. Cytotherapy, 19(12), 1426-1437.
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5

Monitoring RTK signaling in HC11 cells

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For RNA interference experiments, HC11 cells were plated at a concentration of 32,000 cells/ml into 96-well plates. After 24 h, the cells were starved in 0% FCS for 18 h. RTK ligands were added to the media and the cells tranferred to IncuCyte ZOOM incubator (Essen BioScience). For experiments testing the effects of lectins, HC11 cells were plated at 10% confluency into 96-well plates. The cells were preincubated in the presence or absence of lectins for 24 h in medium with 1% FCS. Before transferring the cells into IncuCyte ZOOM, fresh serum-free medium supplemented with RTK ligands and lectins or not was exchanged to the cells. IncuCyte ZOOM Software was used to determine the number of dying cells and confluency from phase contrast images. The acquired time series were normalized to have the same median value at initial time point and fitted with quadratic regression curves. The presented curves indicate the median values and the whiskers the standard errors of mean.
Vaparanta K., Jokilammi A., Tamirat M., Merilahti J.A., Salokas K., Varjosalo M., Ivaska J., Johnson M.S, & Elenius K. (2022). An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation. Nature Communications, 13, 6953.
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