The cells were treated as indicated, and after 48 h, the cells were fixed with 4% paraformaldehyde for 20 min. After being permeabilized with 0.2% TritonX-100 for 20 min, the samples were blocked by BSA at 37 ℃ for 1 h. Then, the cells were incubated with antibodies against ATF6 (1:100, Proteintech) and Col-2 (1:500, Novus) at 4 ℃ overnight. The next day, the cells were incubated with fuorescently labelled goat anti-rabbit or anti-mouse IgG (1:100, Abbkine) for 1 h at 37 ℃. The nuclei were stained with DAPI. The images were taken using a fuorescence microscope (ZEISS Vert. A1) and analysed with the ImageJ software.
Goat anti rabbit or anti mouse igg
Manufactured by Abbkine
Goat anti-rabbit or anti-mouse IgG is a secondary antibody used for the detection and quantification of rabbit or mouse primary antibodies. It is produced by immunizing goats with rabbit or mouse immunoglobulin G (IgG) and purifying the resulting antibodies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using goat anti rabbit or anti mouse igg
1
Immunofluorescence Analysis of ATF6 and Col-2
2
Immunofluorescence Analysis of ATF6 and Col-2
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The cells were treated as indicated, and after 48 h, the cells were fixed with 4% paraformaldehyde for 20 min. After being permeabilized with 0.2% TritonX-100 for 20 min, the samples were blocked by BSA at 37 ℃ for 1 h. Then, the cells were incubated with antibodies against ATF6 (1:100, Proteintech) and Col-2 (1:500, Novus) at 4 ℃ overnight. The next day, the cells were incubated with fuorescently labelled goat anti-rabbit or anti-mouse IgG (1:100, Abbkine) for 1 h at 37 ℃. The nuclei were stained with DAPI. The images were taken using a fuorescence microscope (ZEISS Vert. A1) and analysed with the ImageJ software.
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