Strains were grown on glass slides with a central depression containing 130 µL M2 medium or linoleic acid-containing M2 medium (0.8 mM) for one day under standard conditions. For the staining of lipid droplets, grown mycelium was incubated with LipidSpot™ 488 (Biotium, Fremont, CA, USA; 70065) for 15 min. Fluorescence microscopic analyses were performed with a fluorescence microscope (DM LB/11888011, Leica, Wetzlar, Germany) and a DFC7000 T camera; image processing was conducted with the corresponding LAS X software from Leica (Leica Microsystems GmbH, Wetzlar, Germany).
Dm lb 11888011
Manufactured by Leica
Sourced in Germany
The DM LB/11888011 is a Leica microscope designed for laboratory use. It is a compact and durable instrument that provides high-quality imaging capabilities for various applications.
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Lab products found in correlation
3 protocols using dm lb 11888011
1
Lipid Droplet Staining in Fungal Mycelia
2
Microscopic Analysis of P. anserina Vacuoles
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For microscopic analysis, P. anserina strains were cultivated on glass slides with a central depression containing M2 medium for 1 day at 27 °C and constant light. For vacuole staining, the strains were treated with 2 μg/mL FMTM 4–64 Dye (Invitrogen, Waltham, MA, USA; T13320) 5 h before microscopic analysis and was transferred to darkness. For visualization and documentation of hyphae, a fluorescence microscope (DM LB/11888011, Leica, Wetzlar, Germany) equipped with the appropriate excitation and emission filters was used.
3
P. anserina Mycelia Fluorescence Microscopy
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P. anserina mycelia were grown on glass slides with a central depression filled with CM agar and incubated for 1 d at 27°C and constant light. Hyphae were visualized and documented using a fluorescence microscope (DM LB/11888011, Leica, Wetzlar, Germany) equipped with the appropriate excitation and emission filters. Light sheet-based fluorescence microscopy (LSFM) and quantification of autophagosomes was performed as described previously.12 (link)
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