Rapamycin s1039

Manufactured by Selleck Chemicals

Sourced in United States

Rapamycin (S1039) is a macrolide compound that inhibits the mammalian target of rapamycin (mTOR) signaling pathway. It is a widely used research tool to study mTOR-related cellular processes.

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Lab products found in correlation

Rhodamine b 83689, by Merck Group (1 mentions) Mg132 s2619, by Selleck Chemicals (1 mentions) 3 ma s2767, by Selleck Chemicals (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sgc 7901, by Shanghai Cell Bank (1 mentions) Mgc 803, by Shanghai Cell Bank (1 mentions) Hgc 27, by Shanghai Cell Bank (1 mentions) Minimum essential medium (mem), by Corning (1 mentions) Bgc 823, by Shanghai Cell Bank (1 mentions) A600669, by Sangon (1 mentions) Sc 47778, by Santa Cruz Biotechnology (1 mentions) Torin 1 s2827, by Selleck Chemicals (1 mentions) Bay 11 7 082 hy 13453, by MedChemExpress (1 mentions) Bovine serum albumin (bsa), by Sangon (1 mentions) Ubiquitin sc 8017, by Santa Cruz Biotechnology (1 mentions) Ly294002 l9908, by Selleck Chemicals (1 mentions) Mk2206 s1078, by Selleck Chemicals (1 mentions) Mg132 bml pi102, by Enzo Life Sciences (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions)

8 protocols using rapamycin s1039

PHBHHx, PVA, and Rapamycin Polymer Synthesis

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Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) (Mw =4 × 10⁵) was donated by Lab of Microbiology, Department of Biological Science and Biotechnology, Tsinghua University (Beijing, P. R. China). PHBHHxPEG (Mw =1.8 × 10⁵) was produced by our group [10 ]. Poly(vinyl alcohol) (PVA) (P1763), poly(DL-lactide) (PLA) (P1691) and rhodamine B (83689) were purchased from Sigma-Aldrich (USA). Rapamycin (S1039) was purchased from selleckchem (USA).

Lu X.Y., Li M.C., Zhu X.L., Fan F., Wang L.L, & Ma J.G. (2014). Microbial synthesized biodegradable PHBHHxPEG hybrid copolymer as an efficient intracellular delivery nanocarrier for kinase inhibitor. BMC Biotechnology, 14, 4.

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Cytokine Induction Assay Protocol

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Reagents were obtained from the following suppliers: 3‐MA (S2767), MG132 (S2619), and rapamycin (S1039) were purchased from Selleck. Poly(I:C) and poly(dA:dT) (Sigma‐Aldrich), DMSO (Sigma, D2650), and HBSS (Gibco, 1441787) were obtained from commercial sources. The enzyme‐linked immunosorbent assay (ELISA) kits for human IFN‐β and IL6 were purchased from Thermo Fisher and Dakewe Biotech.

He X., Zhu Y., Zhang Y., Geng Y., Gong J., Geng J., Zhang P., Zhang X., Liu N., Peng Y., Wang C., Wang Y., Liu X., Wan L., Gong F., Wei C, & Zhong H. (2019). RNF34 functions in immunity and selective mitophagy by targeting MAVS for autophagic degradation. The EMBO Journal, 38(14), e100978.

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Gastric Cancer Cell Line Culture

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Five human gastric cancer cell lines AGS, HGC27, SGC7901, BGC823 and MGC803 were obtained from Shanghai Cell Bank of Chinese Academy of Sciences. Cells were cultured at 37°C with 5% CO₂ and maintained in Modified Eagle's medium (MEM, Corning, US) supplemented with 10% fetal bovine serum and 100 μg/mL penicillin/streptomycin. Rapamycin (S1039) was purchased from Selleck Chemicals, US.

Dou N., Yu S., Ye X., Yang D., Li Y, & Gao Y. (2016). Aberrant overexpression of ADAR1 promotes gastric cancer progression by activating mTOR/p70S6K signaling. Oncotarget, 7(52), 86161-86173.

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Combination Cytotoxicity Assay of 5-FU and Rapamycin

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5-Fluorouracil (5-FU, S1209) and inhibitors of mTOR (rapamycin, S1039) were purchased from Selleck (Houston, TX, USA). The working concentration of 5-FU on HCT116 was 0–10 μg/ml. And the working concentration of 5-FU on SW620 was 0–20 μg/ml. The working concentration of rapamycin was 1 μM. For this assay, 1 × 10⁴ cells were seeded into 96-well plates and cultured with different concentrations of 5-FU for 24 h. And the cell viability was also measured by CCK-8, we detected OD450 at 24 h, OD450 of cells treating with 5-FU was normalized by OD450 of cells without treatment to get the cell viability (%). The cell viability was calculated according to the following formula: cell viability (%) = (OD450 of cells treating with 5-FU)/(OD450 of cells without treatment) * 100. The effect of compound treatment was confirmed by Western blotting.

Su W., Feng B., Hu L., Guo X, & Yu M. (2022). MUC3A promotes the progression of colorectal cancer through the PI3K/Akt/mTOR pathway. BMC Cancer, 22, 602.

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Sendeng-4 Signaling Pathway Analysis

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Sendeng-4 was purchased from the Chinese National Institute (Beijing, China); antibodies for AKT (4685), phosphor-AKT (4060), ERK (4695), phosphor-ERK (4370), LC3B (ab192890), Beclin-1 (3495), β-actin (3700), JNK (ab179461), phosphor-JNK (ab124956), p38 (8690), phosphor-p38 (4511), BAX (89477), BCL2 (15071) were purchased from Abcam and Cell Signaling Technology. CCK-8 kit for cell proliferation detection, enhanced chemiluminescent (ECL) kit for western blot, and bicinchoninic acid (BCA) kit for protein quantitation were all purchased from Helix Biotech. Rapamycin (S1039) and 3-MA (66389) were purchased form the Selleck.

Du R., Zhao P., Wu S., Gao Y., Wu R., Yang M., Song W, & Gao H. (2021). Sendeng-4 Suppressed Melanoma Growth by Induction of Autophagy and Apoptosis. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 5519973.

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Antibodies and Pharmacological Inhibitors

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The antibodies against phosphorylated p-rpS6 (4858), rpS6 (2217), MTOR (2972), (p)-MTOR (5536), RELA (8242), p-RELA (4812) were sourced from Cell Signaling Technology. The antibody against MMP12 (ab52897) was acquired from Abcam, and the one against ACTB (sc-47778) was obtained from Santa Cruz Biotechnology. All these antibodies were diluted in accordance with the manufacturer’s instructions using either 5% nonfat dried milk (Sangon Biotech, A600669) or 5% BSA (Sangon Biotech). For flow cytometry, we used anti-mouse CD45 PE-Cyanine7 (25-0451-82), anti-mouse CD11c+ FITC (11-0114-82), anti-human/mouse phospho-MTOR (S2448) eFluor 450 (48-9718-42), and anti-human/mouse phosphor-rpS6 (S235/S236) APC (17-9007-42) antibodies from eBioscience. The anti-mouse Siglec-F PE (562068) antibody was purchased from BD Biosciences. BAY 11-7082 (HY-13453) was obtained from Medchem Express and was used at a final concentration of 2.5 μM in the culture medium. Rapamycin (S1039) and Torin 1 (S2827) were sourced from Selleck, and their final concentrations were 1.25 and 125 nM, respectively.

Dong L., Wang Y., Chen H., Li Z., Xu X., Zhou J., Shen H, & Chen Z. (2024). MTOR Suppresses Cigarette Smoke-Induced Airway Inflammation and MMP12 Expression in Macrophage in Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease, 19, 269-279.

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Investigating Cellular Signaling Pathways

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Slug (9585S), CUL1 (4995S), NEDD8 (2745S), p-AKT (ser473) (9271S), phospho-mTOR (ser2448) (2971S), E-Cadherin (3195S), Phospho-STAT3 (9145S), Phospho-Smad2/3 (8828S), Phospho-ERK (9101S), and antibodies of EMT markers (ZO-1, Vimentin, ZEB1, N-cadherin, Claudin-1, β-Catenin, Snail) (Epithelial–Mesenchymal Transition (EMT) Antibody Sampler Kit 9782T) were purchased form Cell Signaling Technology (Danvers, MA); β-tubulin (sc-166729), p53 (sc-126), p21 (sc-6246), MDM2 (sc-965), and Ubiquitin (sc-8017) antibodies from Santa Cruz Biotechnology (Dallas, TX). MLN4924 was synthesized, as previously described [26 (link)]. MG132 (BML-PI102) (Enzo Life Sciences, Plymouth Meeting, PA, USA), Cycloheximide (C1299) (Sigma Aldrich, St. Louis, MO, USA), LY294002 (L9908), MK-2206 (s1078), and Rapamycin (s1039) (Selleck Chemicals, Houston, TX, USA) were purchased from the indicated company.

Kim Y., Park J.B., Fukuda J., Watanabe M, & Chun Y.S. (2021). The Effect of Neddylation Blockade on Slug-Dependent Cancer Cell Migration Is Regulated by p53 Mutation Status. Cancers, 13(3), 531.

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Oligonucleotide Synthesis and Characterization

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The oligonucleotides used in the current work are listed in Supplementary Table 1 and were purchased either from Sigma Aldrich, USA or IDT in lyophilized form. Nuclease-free water was used to prepare stocks of 100 µM. Rapamycin (S1039) from Selleck chemical LLC and TMPyP4-tosylate (4253) from Tocris were procured without further purification. Bis-4,3 was provided as a gift by Dr Prathap Reddy Patlolla of IIT Gandhinagar.

Majumder P., Shukla C., Arya A., Sharma S, & Datta B. (2024). G-quadruplexes in MTOR and induction of autophagy. Scientific Reports, 14, 2525.

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