Genomic DNA was extracted from 200 µl of EDTA-anti-coagulated whole blood, using a QIAamp® DNA Blood Mini kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions. SNPs were genotyped with TaqMan allele-specific PCR amplification technology on an ABI PRISM 7900 Sequence Detection System and analyzed with the Sequence Detection Software (SDS 2.1) (both from Applied Biosystems, Foster City, CA, USA) by laboratory personnel blinded to clinical status. In order to assess genotyping reproducibility, initially observed SNP allelic discrimination curves of all genotypes were confirmed by direct DNA sequencing on an ABI PRISM 3100 genetic analyzer (Applied Biosystems).
Sequence detection software sds 2.1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Sequence Detection Software (SDS 2.1) is a software application developed by Thermo Fisher Scientific for real-time PCR data analysis. The software's core function is to capture, analyze, and manage real-time PCR data generated from compatible instruments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sequence detection software sds 2.1
1
DNA Extraction and Genotyping from Whole Blood
2
Genotyping of Genetic Variants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic DNA extraction was carried out from 200 μl of EDTA-anticoagulated whole blood using a QIAamp® DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s protocol. The EVER1/EVER2 (rs2290907 and rs16970849) and FAS-670 (rs1800682) SNPs were genotyped with TaqMan allele-specific PCR amplification technology on an ABI PRISM 7900 Sequence Detection System (Applied Biosystems, Foster City, USA) and analysed with the Sequence Detection Software (SDS 2.1, Applied Biosystems, Foster City, USA). Initially observed SNP allelic discrimination curves of all genotypes were confirmed by direct DNA sequencing on an ABI PRISM 3100 genetic analyser (Applied Biosystems, Foster City, USA).
Regarding the linkage-disequilibrium (LD) with other SNPs, according to the International HapMap Project, the FAS gene has three LD blocks and rs1800682 is located in the first LD block and therefore is in high LD with other SNPs within this block. Similarly, the TNRC6C gene has two LD blocks and rs2290907 is located in the second large block. Finally, TMC8 gene has four LD blocks and rs16970849 is located in the last one.
Regarding the linkage-disequilibrium (LD) with other SNPs, according to the International HapMap Project, the FAS gene has three LD blocks and rs1800682 is located in the first LD block and therefore is in high LD with other SNPs within this block. Similarly, the TNRC6C gene has two LD blocks and rs2290907 is located in the second large block. Finally, TMC8 gene has four LD blocks and rs16970849 is located in the last one.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!