The largest database of trusted experimental protocols

4 protocols using gallocatechin gallate gcg

1

Purification and Antioxidant Evaluation of Tea Catechins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eight tea catechin derivatives – (+)-catechin(C), (−)-epicatechin (EC), (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (+)-catechin gallate (CG), (−)-epicatechin gallate (ECG), (−)-gallocatechin gallate (GCG), and (−)-epigallocatechin gallate (EGCG) – were purchased from Sigma (St. Louis, MO, USA). The compounds, purified using the HPLC system, were of analytical grade. We purchased 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ), and all other chemicals from Sigma Aldrich (St. Louis, MO, USA) and all were of analytical grade.
+ Open protocol
+ Expand
2

Comprehensive Analytical Standards Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The standards for gallic acid (GA), (+)–catechin (C), (−)–epicatechin (EC), (−)–epigallocatechin (EGC), (−)–gallocatechin gallate (GCG), (−)–epigallocatechin gallate (EGCG), (−)–epicatechin gallate (ECG), and caffeine were purchased from Sigma–Aldrich (Darmstadt, Germany). The mixture of amino acids standards was purchased from Sykam (Munich, Germany). n–Alkanes were purchased from Sigma–Aldrich (Darmstadt, Germany). Aroma standards including ethyl decanoate (99 %), benzaldehyde (99.5 %), octanal (99 %), benzyl alcohol (99.5 %), phenylacetaldehyde (95 %), linalool (98 %), nonanal (96 %), terpineol (95 %), geraniol (99 %), (Z)–3–hexenyl hexanoate (98 %), β–ionone (97 %), cedrol (98 %), and methyl jasmonate (98 %), were purchased from Aladdin (Shanghai, China). Aroma standards including hexanal (98 %), (Z)–3–hexenyl acetate (97 %), linalool oxide mixture (97 %), 2–phenylethyl alcohol (98 %), methyl salicylate (99 %), indole (99 %), (Z)–jasmone (92 %), α–ionone (90 %), nerolidol (97 %), and (Z)–3–hexenyl benzoate (98 %) were purchased from Tokyo Chemical Industry Co., ltd (Tokyo, Japan). Aroma standard ocimene (90 %) was purchased from Yuanye Company (Shanghai, China).
+ Open protocol
+ Expand
3

Production and Characterization of HTGT and EMIQ

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heat transformed green tea (HTGT: Lot #. 180426) was produced as described previously [16 (link)]. Briefly, dried leaf of green tea was extracted with 50% ethanol and heated at 115 °C for 5 h to facilitate the conversion of epigallocatechin gallate to Gallocatechin gallate (GCG). Enzymatically-modified isoquercitrin (EMIQ: Lot #. 20191102) were prepared by enzymatic hydrolysis of rutin, followed by transglycosylation reaction [17 (link)]. HTGT and EMIQ were provided by Amorepacific Corp. Gallocatechin gallate (GCG) and mirabegron were purchased from Sigma (USA).
+ Open protocol
+ Expand
4

Tea Catechins and Theaflavins Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
The catechins including (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (+)-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechin gallate (EGCG), (−)-gallocatechin gallate (GCG), (−)-epicatechin gallate (ECG), (−)-catechin gallate (CG), caffeine, l-theanine and eighteen amino acid standards (kit) were purchased from Sigma (St. Louis, MO, USA). Four theaflavin monomers including theaflavin (TF1, 95%), theaflavin-3-gallate (TF2A, 92%), theaflavin-3′-gallate (TF2B, 90%) and theaflavin-3, 3′-digallate (TF3, 90%) were prepared according to the method developed in our lab, and their purities were determined by high-performance liquid chromatography-ultraviolet-mass spectrum (HPLC-UV-MS), with the standard samples from sigma company.12 (link) Methanol and acetonitrile of HPLC grade were purchased from Tianjin Shield Company (Tianjin, China). Other reagents of analytical reagent grade were purchased from National Medicine Chemical Reagent Co., Ltd. (Shanghai, China).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!