TR146 cell was seeded in a 12-well Transwell insert (Corning Inc., Corning, NY, USA) at a density of 5 × 104 cells per cm2, and the medium was changed every 2 days for 28–30 days. The experiment was conducted from apical to basolateral and HBSS-HEPES buffer was used as media. Forty micrograms of sCT and 1.22, 6.10, and 12.2 μM of Penetratin were added to apical chamber and kept at 37 °C. 500 μL of sampling was obtained at 0.5, 1, 2, 4 and 8 h. Transepithelial electrical resistance (TEER) value was measured before and after the experiment. TEER was calculated with the following Equation (1), where Rinsert with cell, Rinsert without cell, and A were insert filter areas. The permeated amount of sCT was analyzed using an sCT ELISA kit (Phoenix Pharmaceuticals, Burlingame, CA, USA):
Sct elisa kit
Manufactured by Phoenix Pharmaceuticals
Sourced in United States
The SCT ELISA kit is a laboratory equipment product designed for enzyme-linked immunosorbent assay (ELISA) testing. It provides the necessary components and protocols to perform quantitative analysis of target analytes in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Snakeskindialysis tubing 3.5k mwco, by Thermo Fisher Scientific (2 mentions)
Transwell insert, by Corning (1 mentions)
L α lecithin, by Merck Group (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay mts kit, by Promega (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Salmon calcitonin sct, by Bachem (1 mentions)
Alexa fluor 647 nhs ester, by Thermo Fisher Scientific (1 mentions)
Tween 80, by Duksan Pure Chemicals (1 mentions)
Salmon calcitonin, by Bachem (1 mentions)
F 12 nutrient mixture ham ham s f 12, by Welgene (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay kit, by Promega (1 mentions)
4 protocols using sct elisa kit
1
Transepithelial Transport Efficiency of sCT
2
Salmon Calcitonin Delivery Using Cys-Penetratin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Salmon calcitonin (sCT) was purchased from Bachem AG (Bubendorf, Switzerland). The peptide CRQIKIWFQNRRMKWKK (Cys-penetratin) was synthesized and purchased from Peptron Co., Ltd. (Daejeon, Republic of Korea). The lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl) butyramide] (MPB-PE) was purchased from Avanti Polar Lipids (Alabaster, AL). L-α-lecithin (97.7% phosphatidylcholine) was purchased from Merck Millipore (Billerica, MA, USA). Tween 80® was purchased from Duksan (Ansan, Republic of Korea). An sCT ELISA kit was purchased from Phoenix Pharmaceuticals (Burlingame, CA, USA). A Ham-F-12 Nutrient mixture (Ham’s F-12) was purchased from Welgene (Gyeongsan-si, Republic of Korea). A CellTiter 96® Aqueous One Solution Cell Proliferation Assay kit (MTS) was purchased from Promega (Madison, WI, USA). Alexa Fluor 647 NHS Ester, SnakeSkinTM dialysis tubing (3.5 K MWCO), fetal bovine serum (FBS), 0.25% trypsin-EDTA, and penicillin/streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and solvents were of reagent grade.
3
Buccal Permeability of sCT Evaluated
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Buccal permeability of sCT was evaluated using the Franz diffusion cell. The permeation area was 2.0 cm2, and the volume of receptor media was 12.5 mL. The receptor was filled with pH 7.4 PBS and stirred at 600 rpm at 37 °C. The Franz diffusion cell was equilibrated for 30 min after mounting the buccal epithelium. Forty micrograms of sCT and of 1.22, 6.10 and 12.2 μM of Penetratin, of which molecular ratios were 1:0.1, 1:0.5 and 1:1 in donor part, respectively, were added to the Franz diffusion cell. Half of a milliliter of sample was obtained at different time intervals (1, 2, 4, and 8 h) from the receptor part and analyzed by a sCT ELISA kit (Phoenix Pharmaceuticals, Burlingame, CA, USA).
4
Salmon Calcitonin Uptake and Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Salmon calcitonin was purchased from Bachem AG (Bubendorf, Switzerland). Penetratin (RQIKIWFQNRRMKWKK) and FITC labeled Penetratin (FITC-Penetratin) were purchased from Peptron Co., Ltd. (Daejeon, Korea). Alexa Fluor 647 NHS Ester, SnakeSkinTM dialysis tubing (3.5 K MWCO), Fetal bovine serum (FBS), 0.25% trypsin-EDTA and penicillin/streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The sCT ELISA kit was purchased from Phoenix Pharmaceuticals (Burlingame, CA, USA). F-12 Nutrient mixture Ham (Ham’s F-12) was purchased from Welgene (Gyeongsan-si, Korea). The CellTiter 96® Aqueous One Solution Cell Proliferation Assay kit (MTS) was purchased from Promega (Madison, WI, USA). All other chemicals and solvents were reagent grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!