Percp anti mouse f4 80

Manufactured by BioLegend

The PerCP anti-mouse F4/80 is a flow cytometry antibody conjugate that specifically binds to the F4/80 antigen expressed on mouse macrophages. It is labeled with the PerCP (Peridinin-chlorophyll-protein) fluorescent dye, which can be detected using a flow cytometer equipped with a 488 nm excitation laser.

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Lab products found in correlation

Fitc anti mouse cd206, by BioLegend (1 mentions) Fitc anti mouse cd206 mmr, by BioLegend (1 mentions) Percp anti mouse cd11b, by BioLegend (1 mentions) Pe anti mouse cd11b, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Apc anti mouse human cd11b, by BioLegend (1 mentions) Apc cyanine7 anti mouse cd86, by BioLegend (1 mentions)

2 protocols using percp anti mouse f4 80

Characterizing Retinal and Choroidal Macrophages

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To examine macrophages in the retina and choroid, cells were prepared from mouse eyes. To collect a sufficient number of ocular in ltrating cells, 50 burns were delivered to mouse eyes by laser. After laser injury, eyes were enucleated at different time points (1, 2, 3, 5, and 7days). The anterior segment (cornea, iris, and lens) was excised and the posterior segment of the eye including sclera, choroid, and retina was disrupted with scissors and then shaken in DMEM (plus 10% FBS (Gibco Laboratories), 100U/ml penicillin, 100μg/ml streptomycin) supplemented with 0.5mg/ml Collagenase type D (11088874103, Boehringer Mannheim) at 37°C for 60min. The supernatants were collected and passed through a mesh. After 3 washes, viable cells were obtained. A total of 12 eyes (6 individual pools) were examined per group. The cells were stained with PE anti-mouse CD11b (557397, BD Pharmingen), FITC anti-mouse CD206 (MMR, 123005; BioLegend) and PE-Cy5 anti-mouse CD80 Abs (15-0801-81, eBioscience).
RAW 264.7 cells were stained with PerCP anti-mouse CD11b (101230, BioLegend), PerCP anti-mouse F4/80 (123006, BioLegend), PE anti-mouse CD80 (12-0801, eBioscience), and FITC anti-mouse CD206 (141704, BioLegend).

Zandi S., Nakao S., Chun K.H., Fiorina P., Sun D., Arita R., Zhao M., Kim E., Schueller O., Campbell S., Taher M., Melhorn M.I., Schering A., Gatti F., Tezza S., Xie F., Vergani A., Yoshida S., Ishikawa K., Yamaguchi M., Sasaki F., Schmidt-Ullrich R., Hata Y., Enaida H., Yuzawa M., Yokomizo T., Kim Y.B., Sweetnam P., Ishibashi T, & Hafezi-Moghadam A. (2015). ROCK-Isoform Specific Polarization of Macrophages Associated with Age-Related Macular Degeneration. Cell reports, 10(7), 1173-1186.

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Macrophage Expression Analysis in Tumor Tissue

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We analyzed the changes in macrophages expression through flow cytometry (FCM). The experiment groups were divided into two sections: the PBS group and the 30 mg/kg CB-NPs group. Tumor tissue was cut and digested to prepare a cell suspension, and the cell count was adjusted to 1 × 10⁶/100 μL. For each group (PBS and CB-NPs, n = 3), 100 μL of the cell suspension was taken, and 2 μL of CD16/32 was added while keeping the samples on ice. Corresponding volumes of fluorescent antibodies were added, which included PerCP anti-mouse F4/80 (123125, Biolegend), APC anti-mouse/human CD11b (101211, Biolegend), APC/Cyanine7 anti-mouse CD86 (105029, Biolegend), and PE/Cyanine7 anti-mouse CD206 (MMR) (141719, Biolegend). The samples were then incubated at room temperature, shielded from light, for 30 min. After incubation, the supernatant was removed by centrifugation, and the cells were resuspended in 200 μL of CSB. The stained cells were analyzed using a flow cytometer (EXFLOW-206, Dakota).

Chen J., Yin M., Yang C., Wang K., Ma L., Yu H., Huang Y., Liu F, & Tang Z. (2024). Therapeutic effects and underlying mechanism of poly (L-glutamic acid)-g-methoxy poly (ethylene glycol)/combretastatin A4/BLZ945 nanoparticles on Renca renal carcinoma. Frontiers in Bioengineering and Biotechnology, 12, 1336692.

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