Hdq p1

Manufactured by Leibniz Institute DSMZ

Sourced in Germany

The HDQ-P1 is a laboratory instrument designed for high-throughput DNA extraction and purification. It utilizes a magnetic bead-based method to efficiently isolate DNA from a variety of sample types. The core function of the HDQ-P1 is to automate the DNA extraction process, ensuring consistent and reliable results.

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Lab products found in correlation

4 protocols using hdq p1

Cell Line Authentication and Maintenance

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All cell lines were obtained from King’s College London Breast Cancer Now Unit, except HDQ-P1, purchased from Leibniz Institute DSMZ. Cell lines were authenticated by short tandem repeat profiling. Cells used once tested negative for mycoplasma and used up to 30 passages. All cell lines were maintained in 5% CO₂ humidified incubator at 37 °C.

Cheung A., Chenoweth A.M., Quist J., Sow H.S., Malaktou C., Ferro R., Hoffmann R.M., Osborn G., Sachouli E., French E., Marlow R., Lacy K.E., Papa S., Grigoriadis A, & Karagiannis S.N. (2022). CDK Inhibition Primes for Anti-PD-L1 Treatment in Triple-Negative Breast Cancer Models. Cancers, 14(14), 3361.

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Cell Line Authentication and Mycoplasma Testing

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Cell lines were obtained from King’s College London (KCL) Breast Cancer Now Unit, except HDQ-P1, purchased from Leibniz Institute DSMZ. Cell lines were authenticated by short tandem repeat profiling. Cells used once tested negative for mycoplasma and used up to 30 passages.

Cheung A., Opzoomer J., Ilieva K.M., Gazinska P., Hoffmann R.M., Mirza H., Marlow R., Francesch-Domenech E., Fittall M., Dominguez Rodriguez D., Clifford A., Badder L., Patel N., Mele S., Pellizzari G., Bax H.J., Crescioli S., Petranyi G., Larcombe-Young D., Josephs D.H., Canevari S., Figini M., Pinder S., Nestle F.O., Gillett C., Spicer J.F., Grigoriadis A., Tutt A.N, & Karagiannis S.N. (2018). Anti-Folate Receptor alpha–directed Antibody Therapies Restrict the Growth of Triple Negative Breast Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(20), 5098-5111.

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Breast Cancer Cell Line Cultivation Protocol

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Human TNBC cell lines MDA-MB-231, MDA-MB-436, MDA-MB-157, HCC1806, HCC1937, Hs578T, HCC38, BT549, HCC1187, and HCC1395 were obtained from American Type Culture Collection (ATCC, Manassas, VA). CAL-51, CAL-85-1, CAL-120, CAL-148 and HDQ-P1 were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany). MDA-MB-468, BT20, and HCC70 were obtained from the University of Colorado Cancer Center (UCCC) Tissue Culture Core laboratory. The MCF-10A cell line was obtained as a kind gift from Traci Lyons, PhD and used as a control. All breast cancer cells were cultured in DMEM supplemented with 10% FBS, 1% penicillin–streptomycin, 1% MEM nonessential amino acids and 1% normocin. All cells were grown in an incubator at 37°C containing 5% CO₂. Cell lines were routinely authenticated by the Barbara Davis Center for Childhood Diabetes Core and screened for mycoplasma every 3 months.

Capasso A., Bagby S.M., Dailey K.L., Currimjee N., Yacob B.W., Ionkina A., Frank J.G., Kim D.J., George C., Lee Y.B., Benaim E., Gittleman B., Hartman S.J., Tan A.C., Kim J., Pitts T.M., Eckhardt S.G., Tentler J.J, & Diamond J.R. (2019). First-in-class phosphorylated-p68 inhibitor RX-5902 inhibits β-catenin signaling and demonstrates anti-tumor activity in triple-negative breast cancer. Molecular cancer therapeutics, 18(11), 1916-1925.

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Cell Culture and Heavy Labeling

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Adherent cells were cultured in Dulbecco’s modified Eagle’s media (Corning) containing 8% fetal bovine serum (HyClone) and penicillin/streptomycin (Sigma Aldrich, 100 U/mL and 100 μg/mL, respectively). Cells were cultured in a humidified incubator at 37 °C with 5% CO₂. MCF7, T47D, HCC1428, BT474, SKBR3, and MDA-MB-361 cells were a gift from Todd Miller (Geisel School of Medicine at Dartmouth). BT549, HCC1806, MDA-MB-468, HCC1954, HeLa, and HCT116 cells were obtained from ATCC. Cal120 and HDQ-P1 cells were obtained from DSMZ. FreeStyle^™ 293-F cells were obtained from Gibco and grown in suspension in Gibco FreeStyle^™ expression media at 37 °C and 5% CO₂. For heavy labeling, HeLa and HCT116 cells were grown in Gibco media supplemented with 100 mg/L ¹³C₆,¹⁵N₄-arginine and 100 mg/L ¹³C₆,¹⁵N₂-lysine (Cambridge Isotope Laboratories) for 6 – 8 doublings. Heavy-labeled cells were checked for the degree of heavy amino acid incorporation and proline conversion prior to use as a standard. All cells were regularly tested for mycoplasma contamination.

Cadavid L.F., Shufflebotham C., Ruiz F.J., Yeager M., Hughes A.L, & Watkins D.I. (1997). Evolutionary instability of the major histocompatibility complex class I loci in New World primates. Proceedings of the National Academy of Sciences of the United States of America, 94(26), 14536-14541.

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