Western blot analyses were performed as described previously [7 (link)]. Briefly, samples (20 μg protein) were subjected to SDS–PAGE. The proteins were transferred onto nitrocellulose membranes, which were probed with the specific antibodies: anti-ZO-1, anti-vimentin, anti-Smad7 and anti-Smurf2 (Santa Cruz, 1:1000), anti-pan-Akt (phospho T308) antibody (Abcam) also known as anti-pAkt, anti-total Akt, (Cell Signaling, 1:1000) and anti-FN (BD Biosciences, 1:1000). A peroxidase-conjugated goat anti mouse IgG (1: 10 000) was used as a secondary antibody. For detection of other proteins or β-actin, the membranes were treated with Stripping buffer and then re-probed with another antibody or β-actin antibody, the latter served as an internal control.
Anti pan akt phospho t308 antibody
Manufactured by Abcam
Sourced in United States
The Anti-pan-Akt (phospho T308) antibody is a primary antibody that specifically recognizes Akt (also known as Protein Kinase B) when phosphorylated at threonine 308. Akt is a serine/threonine protein kinase that plays a key role in multiple cellular processes such as cell growth, proliferation, and survival. This antibody can be used to detect and study the phosphorylation status of Akt in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti fn, by BD (1 mentions)
Anti vimentin, by Santa Cruz Biotechnology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Anti smurf2, by Santa Cruz Biotechnology (1 mentions)
Anti zo 1, by Santa Cruz Biotechnology (1 mentions)
Anti total akt, by Cell Signaling Technology (1 mentions)
Anti smad7, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti lc3b, by Cell Signaling Technology (1 mentions)
2 protocols using anti pan akt phospho t308 antibody
1
Western Blot Protein Analysis Protocol
2
Western Blot Antibody Panel for Signaling Pathway Analysis
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The following antibodies were used for Western blot analyses.
Mouse monoclonal: anti-PKBα/Akt from BD Transduction (Lexington, KY, USA); anti-tubulin and anti-vinculin from Sigma; anti-LAMP2 and anti MPK3/DUSP6 from Abcam (Cambridge, UK); anti-pan-Ras from Calbiochem (San Diego, CA, USA); anti-p62 from MBL (Woburn, MA, USA).
Rabbit monoclonal: anti-phospho-IGF1-IRβ (Tyr1135/1136)/InsRβ (Tyr1150/1151) (19H7) and anti-phospho-PDGFRα (Tyr849)/PDGFRβ (Tyr857) from Cell Signaling Technology (Beverly, MA, USA).
Rabbit polyclonal: anti-SYT (H-80) and anti-InsRβ (C-19) from Santa Cruz Biotechnology. Anti-cleaved PARP (Asp214), anti-phospho-p44/42 MAPK (Thr202/Tyr204), anti-p44/42 MAPK, anti-phospho-Akt (Ser473), anti-cleaved Caspase-3 (Asp175), and anti-LC3B from Cell Signaling Technology; anti-PDGFRα from Upstate Biotechnology (Lake Placid, NY, USA); anti-pan-Akt phosphoT308 antibody from Abcam; anti-actin from Sigma.
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