Massarray rt 3

Peripheral venous blood (2 ml) was collected from each participant, and then genomic DNA was isolated using a WizardVR genomic DNA purification kit (Promega, Madison, U.S.A.) following the supplier’s manual. The concentration and purity of the genomic DNA were estimated on NanoDrop using two optical density wavelengths 260 and 280 nm.
Genotyping was done by MALDI-TOF-MS using a MassARRAY system (Sequenom, San Diego, CA, U.S.A.) as previously described [13 (link)]. Complete genotyping reactions were spotted on to a 384-well spectroCHIP (Sequenom) using MassARRAY nanodispenser (Sequenom) and analyzed by MALDI-TOF-MS. Genotype callings were done in real time with MassARRAY RT 3.1 and analyzed on MassARRAY Typer 4.0 (both Sequenom). For quality control, 10% of the randomly selected samples were analyzed repeatedly.

Venous blood (2 mL each) was sampled in tubes containing ethylenediamine-tetraacetic acid (EDTA) and stored at –80°C before use. Genomic DNA was extracted using a QIAamp DNA blood mini kit (Qiagen, Germany), and the concentration and purity were estimated using NanoDrop (Thermo Electron Corp., USA) at two absorbance wavelengths of 260 and 280 nm. Genotyping was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) using a MassARRAY system (Sequenom, USA). Completed genotyping reactions were spotted onto a 384-well spectroCHIP (Sequenom) using a MassARRAY nanodispenser (Sequenom) and analyzed by MALDI-TOFMS. Genotype calling was done in real time with MassARRAY RT 3.1 (Sequenom) and analyzed on MassARRAYTyper 4.0 (Sequenom). For quality control, 10% of randomly-selected samples were analyzed repeatedly.

In brief, 2mL of peripheral blood was collected in vacuum tubes containing 5% EDTA and frozen at -80 °C until use. Genomic DNA was extracted using a DNA Purification Kit (Tiangen Biotech) according to the manufacturer’s instructions. and the concentration and purity were estimated using NanoDrop at two optical density (OD) wavelengths 260 and 280 nm. Genotyping was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) using a MassARRAY system (Sequenom, San Diego, CA, USA). Completed genotyping reactions were spotted onto a 384-well spectroCHIP (Sequenom) using a MassARRAY nano dispenser (Sequenom) and analyzed by MALDI-TOFMS. Genotype were called in real time on MassARRAY RT 3.1 (Sequenom) and analyzed on MassARRAY Typer 4.0 (Sequenom). For quality control, 10% of randomly-selected samples were analyzed repeatedly.