The percentage of apoptosis was evaluated by using an Annexin V-PE/7-AAD or Annexin V-FITC/PI staining detection Kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer's protocol. Cells were treated with agents for indicated time, and then harvested and washed two times with PBS. Pellets were collected and resuspend in PBS, and then stained with PE Annexin V and 7-AAD or Annexin V and PI for 15 minutes in the dark. Data were then obtained by a FACSCalibur (Becton Dickinson, USA).
Annexin 5 fitc pi staining detection kit
Manufactured by BD
Sourced in United States
The Annexin V-FITC/PI staining detection Kit is a laboratory reagent used for the detection and quantification of apoptosis in cell samples. The kit contains Annexin V-FITC, a fluorescent conjugate of the Annexin V protein, and Propidium Iodide (PI), a fluorescent dye that binds to DNA. This combination allows for the identification of cells undergoing early and late stages of apoptosis.
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Lab products found in correlation
2 protocols using annexin 5 fitc pi staining detection kit
1
Apoptosis Evaluation by Flow Cytometry
2
Apoptosis Evaluation of Compound 1a in OS-732 Cells
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After the
treatment of compound1a , the apoptotic percentage of
the OS-732 cells was determined
through the flow cytometer using Annexin V-FITC/PI staining detection
kit (BD Biosciences, New Jersey). This preformation was finished totally
in accordance with the protocols’ guidance with minor modification.
In short, the OS-732 cells in the logarithmic growth phase were collected
and then planted into six-well plates at 1 × 105 cells/well
ultimate destiny. After incubation at 5% CO2 and 37 °C
conditions overnight, the cells were then treated with1a for 24 h. Afterward, the OS-732 cells were harvested, cleaned, and
resuspended in a 1× binding buffer. Subsequently, 5 μL
of Annexin V-FITC and 5 μL of propidium iodide (PI) solution
were added into the cell suspension and then inoculated in darkness
for 20 min. The apoptosis of the cell was determined in the flow cytometry
at 525/625 and 488 nm. All of the studies were implemented
in triplicate.
treatment of compound
the OS-732 cells was determined
through the flow cytometer using Annexin V-FITC/PI staining detection
kit (BD Biosciences, New Jersey). This preformation was finished totally
in accordance with the protocols’ guidance with minor modification.
In short, the OS-732 cells in the logarithmic growth phase were collected
and then planted into six-well plates at 1 × 105 cells/well
ultimate destiny. After incubation at 5% CO2 and 37 °C
conditions overnight, the cells were then treated with
resuspended in a 1× binding buffer. Subsequently, 5 μL
of Annexin V-FITC and 5 μL of propidium iodide (PI) solution
were added into the cell suspension and then inoculated in darkness
for 20 min. The apoptosis of the cell was determined in the flow cytometry
at 525/625 and 488 nm. All of the studies were implemented
in triplicate.
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