Rabbit anti phospho mek1 2 ser217 221

Manufactured by Cell Signaling Technology

Rabbit anti phospho-MEK1/2 (Ser217/221) is a primary antibody that specifically recognizes the phosphorylated forms of MEK1 and MEK2 proteins at serine residues 217 and 221. This antibody can be used to detect the activation state of the MEK proteins, which are key components of the MAPK signaling pathway.

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Lab products found in correlation

Rabbit anti actin, by Merck Group (1 mentions) Anti rabbit irdye 680rd, by LI COR (1 mentions) Rabbit anti mek1 2, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Anti mouse irdye 800cw, by LI COR (1 mentions) Mouse anti mmp7, by Santa Cruz Biotechnology (1 mentions) Mouse anti mmp9, by Santa Cruz Biotechnology (1 mentions) Anti phospho akt ser473 antibody, by Cell Signaling Technology (1 mentions) Mouse anti β actin antibody, by Proteintech (1 mentions) Mouse anti gapdh, by Proteintech (1 mentions) Alexa fluor 594 labeled secondary antibody, by Thermo Fisher Scientific (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Dynasore, by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) Mouse anti lamp2, by Santa Cruz Biotechnology (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Dyngo 4a, by Selleck Chemicals (1 mentions) Rabbit anti eea1, by Santa Cruz Biotechnology (1 mentions) Mouse anti α tubulin antibody, by Merck Group (1 mentions)

Spelling variants of this product

MEK1/2 (167 citations) Phospho-MEK1/2 (80 citations) Anti-MEK1/2 (56 citations) Anti-phospho-MEK1/2 (41 citations) Phospho-MEK1/2 (Ser217/221) (19 citations) Rabbit anti-MEK1/2 (8 citations) Rabbit anti-phospho-MEK1/2 (6 citations) Phospho-MEK1 (5 citations) Anti-phospho-MEK1/2 (Ser217/221) (4 citations) Rabbit phospho-MEK1/2 (Ser217/221) (2 citations)

2 protocols using rabbit anti phospho mek1 2 ser217 221

Molecular Markers in Neurodegeneration

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We used the following primary antibodies: rabbit anti-mBdnf and proBdnf (Santa Cruz Biotechnology, sc-546), rabbit anti phospho-MEK1/2 (Ser217/221) (Cell Signaling Technology, #9121), rabbit anti MEK1/2 (Cell Signaling Technology, #9122), rabbit anti-Actin (A2066; Sigma-Aldrich), rabbit anti-Hprt (ab10479; Abcam), rabbit anti-Gapdh (Cell Signaling; 2118), HRP anti-rabbit (P0448; Dako), mouse anti-Mmp7 (Santa Cruz; sc-515703), mouse anti-Mmp9 (Santa Cruz; sc-393859), mouse anti-Mmp3 (Santa Cruz; sc-21732), mouse anti-Furin (Santa Cruz; sc-133142), mouse anti-Plasminogen (Santa Cruz; sc-376324), mouse anti-Timp1 (Santa Cruz; sc-365905), rabbit anti-PC1/3 (Cell Signaling; 11914), IRDye 800CW anti-mouse (LI-COR; 925–32210) and IRDye 680RD anti-rabbit (LI-COR; 925–68071)

Spychala A, & Rüther U. (2019). FTO affects hippocampal function by regulation of BDNF processing. PLoS ONE, 14(2), e0211937.

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Antibodies and Inhibitors for EGFR Signaling

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Mouse anti-EGFR (R1), mouse anti-RTN3, mouse anti-LAMP2, rabbit anti-EEA1, and rabbit anti-EGFR (1005) antibodies were purchased from Santa Cruz. Mouse anti-Ubiquitin (P4G7) antibody was obtained from Covance. Rabbit anti-phospho-MEK1/2 (Ser217/221) and anti-phospho-AKT (Ser473) antibodies were obtained from Cell Signaling Technology. Mouse anti-GAPDH and mouse anti-β-Actin antibodies were purchased from Proteintech (Wuhan, China). Mouse anti-α-Tubulin antibody was obtained from Sigma. Goat anti-rabbit and anti-mouse IRDye secondary antibodies (infrared-labeled) were purchased from LICOR. Alexa Fluor 488-labeled and Alexa Fluor 594-labeled secondary antibodies were obtained from Invitrogen. Gefitinib, lapatinib, filipin, and dyngo-4a were purchased from Selleck. EGF was purchased from PeproTech (USA). Cycloheximide was obtained from MP Biologicals. 17-AAG was purchased from Cell Signaling Technology. Chloroquine, puromycin, and dynasore were obtained from Sigma.

Wang T., Zhang J., Wang S., Sun X., Wang D., Gao Y., Zhang Y., Xu L., Wu Y., Wu Y., Liu F., Liu X., Liu S., Zhang Y., Wang Y., Zou L, & Liu H. (2018). The exon 19-deleted EGFR undergoes ubiquitylation-mediated endocytic degradation via dynamin activity-dependent and -independent mechanisms. Cell Communication and Signaling : CCS, 16, 40.

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