Affinipure goat anti rabbit igg

Paraffin-embedded mouse tumour metastatic tissues and clinical GC tissues were cut into 5-μm-thick sections, placed on glass slides and stained with H&E or subjected to IHC analysis. The tissue sections were deparaffinized and subjected to antigen retrieval using 0.01 m citric acid buffer (pH 6.0) at 95m °C for 15 min and incubated overnight at 4 °C with primary antibodies against IRF-1 (Abcam, ab186384, Cambridge, UK, 1:50 dilution), β-catenin (Proteintech, 51067–2-AP, Wuhan, China, 1:50 dilution) and C-Myc (Proteintech, 10828–1-AP, Wuhan, China, 1:50 dilution). After three washes with Tris-buffered saline, the sections were incubated with horseradish peroxidase-conjugated Affinipure goat anti-rabbit IgG (Abcam, ab6795, Cambridge, UK, 1:100 dilution) for 1 hour at room temperature. The IHC results were scored by two independent observers.