N hydroxysulfosuccinimide sulfo nhs

PEG (M_w = 1.5 KDa), PCL (M_w = 10 KDa), poly(vinyl alcohol) ((PVA) M_w = 13–23 KDa, 87–89% hydrolyzed), N-(ethylcarbonimidoyl)-N,N-dimethylpropane-1,3-diamine monohydrochloride (EDAC), N-hydroxysulfosuccinimide (sulfo-NHS), 2-methoxyethylmethyl (MEM), foetal bovine serum (FBS), penicillin/streptomycin, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS), N,N-dimethylformamide (DMF), ethyl acetate, and lactose monohydrate were obtained from Sigma-Aldrich (South Africa). Mox was purchased from DB Fine Chemicals (South Africa) as the chloride salt. Madin-Darby bovine kidney (MDBK) cells stock, frozen in liquid nitrogen, ATCC no. CCL-22 passage: 46 were commercially obtained from VACSERA, Giza, Egypt. Alcohol free and drug free healthy human male donors' blood was used. All aqueous experiments were carried out in freshly prepared phosphate-buffered saline ((PBS) 137 mM NaCl; 2.7 mM KCl; 4.3 mM Na₂HPO₄; 1.47 mM KH₂PO₄ adjusted to a final pH of 7.4). Ultrapure water with resistivity of 18 MΩ cm was used in all aqueous preparations. Bacterium strains were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

NaBH₄ (Merck, 99%), tellurium powder (Merck, 99%), CdCl₂, thiourea, CdSO₄, thioglycolic acid (TGA) (Merck, 98%), Na₂S₂O₃ (Merck, 99%) were obtained from Merck Inc (Darmstadt, Germany, http://www.merck.de). Acetic acid, sodium acetate trihydrate, potassium dihydrogen phosphate, and sodium hydroxide were purchased from Sigma Aldrich (St. Louis, MO, USA, https://www.sigmaaldrich.com/). 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS) were also obtained from Sigma Aldrich.
The NMP22 antibody (anti-NMP22) and NMP22 were purchased from DNA biotech Co. (Tehran, Iran, http://dnabiotech.ir/). Bovine serum albumin (BSA) was acquired from TAKARA Bio Inc. (http://www.takara-bio.com/). Ultrapure water was used in all syntheses. All reagents were of scientific reagent grade, and used as received without further purification. All experiments on the human samples were performed in accordance with the Declaration of Helsinki and approved by the ethics committee at the University of Tehran. Informed consents were obtained from human participants of this study.

Ethanolamine, gold urchin, nanohorn, 16-mercaptoundecanoic acid, 3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysulfosuccinimide (sulfo-NHS) and APTMS were received from Sigma Aldrich (USA). Aβ (1–42) was purchased from DgPeptides Co., Ltd, China. Anti- Aβ antibody was obtained from Abcam (England). The aptamer sequence (5′-SH-GCCTGTGTTGGGGCGGGTGCG) was reported by Tsukakoshi et al and synthesized commercially (Apical Scientific, Malaysia).26 (link) Artificial CSF was prepared by mixing 3.0 mM KCl, 150 mM NaCl, 0.8 mM MgCl₂·6H₂O, 1 mM phosphate and 1.4 mM CaCl₂·2H₂O.27 (link),28 (link)

Chitosan (low molecular weight), curcumin (85% pure), acetic acid, l-ascorbic acid, 2,2′-diphenylpicrylhydrazyl (DPPH), dichloromethane, glutaric anhydride, methanol (HPLC grade), 4-dimethylaminopyridine (DMAP), triethylamine, N-hydroxysulfosuccinimide (sulfo-NHS), 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), diethylether, and 10× phosphate-buffered saline (PBS) were purchased from Sigma Aldrich (St. Louis, MO, USA) and used as received unless otherwise noted. Magnesium sulfate was purchased from Fisher Scientific (Pittsburgh, PA, USA).