Enrofloxacin lactate (EFX) (99.0 %, purity) was purchased from HETIAN Biotech Co., LTD (Zhengzhou). Copper sulfate (Cu) (99.0 %, purity) was purchased from YONGDA Chemical Reagent Co., LTD (Tianjin). LB broth agar was purchased from Sangon Biotech (Shanghai) Co., Ltd. Other chemicals used for the culture medium, including glucose, sodium chloride, magnesium sulfate, ferrous sulfate, dipotassium phosphate, and sodium nitrate were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd.
The experiments were performed with topsoil (0–20 cm) from a peanut experimental field. The soil samples classified as Argosols were collected from Shandong Agricultural University in Taian, China (117.16°E, 36.16°N) during the slack farming seasons in November, 2012. This soil had never been treated with organic fertilizer or exposed to heavy metals. The soil samples were air-dried and ground to pass a 2-mm sieve to remove stones and plant roots. Soil physical and chemical parameters were measured according to the soil agro-chemistrical analysis methods46 .
The experiments were performed with topsoil (0–20 cm) from a peanut experimental field. The soil samples classified as Argosols were collected from Shandong Agricultural University in Taian, China (117.16°E, 36.16°N) during the slack farming seasons in November, 2012. This soil had never been treated with organic fertilizer or exposed to heavy metals. The soil samples were air-dried and ground to pass a 2-mm sieve to remove stones and plant roots. Soil physical and chemical parameters were measured according to the soil agro-chemistrical analysis methods46 .