Piperaquine tetraphosphate tetrahydrate (MW 999.55, purity 99%) was purchased from A.K.Scientific Inc. (Union City, CA, USA). The internal standard (IS) piperaquine-d6 (PQ-d6 , MW 541.55, isotopic purity ≥ 99%) was purchased from AlSAchim, SAS (IllKirch, France). Benzalkonium chloride (BAK) was obtained from Alfa Aesar Co. Trifluoroacetic acid (TFA) and formic acid (FA) (Optima™ LC/MS grade), acetonitrile (MeCN), methanol (MeOH), and other common solvents (HPLC grade) were purchased from Fisher Scientific Co. (Fair Lawn, NJ, USA). Blank human plasma (K2EDTA added as anticoagulant) was obtained from Biological Specialty Co (Colmar, PA, USA); 10% MeCN 0.5% FA solution was prepared by adding 5 mL MeCN and 250 μL FA to 45 mL water (HPLC grade). Mobile phase A (20 mM NH4FA, 0.14%TFA) was prepared by adding 1.262 g NH4 FA and 1.4 mL TFA in 1 L water. Mobile phase B (0.1%TFA) was prepared by mixing 200 mL MeCN, 800 mL methanol and 1 mL TFA in 1 L glass bottle.
Benzalkonium chloride bak
Manufactured by Thermo Fisher Scientific
Sourced in United States
Benzalkonium chloride (BAK) is a type of quaternary ammonium compound commonly used as a preservative and disinfectant in various laboratory applications. It functions as a cationic surfactant, exhibiting antimicrobial properties against a wide range of microorganisms.
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2 protocols using benzalkonium chloride bak
1
Quantification of Piperaquine in Plasma
2
Quantification of Piperaquine in Plasma
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Piperaquine tetraphosphate tetrahydrate (MW 999.55, purity 99%) was purchased from A.K.Scientific Inc. (Union City, CA, USA). The internal standard (IS) piperaquine-d6 (PQ-d6 , MW 541.55, isotopic purity ≥ 99%) was purchased from AlSAchim, SAS (IllKirch, France). Benzalkonium chloride (BAK) was obtained from Alfa Aesar Co. Trifluoroacetic acid (TFA) and formic acid (FA) (Optima™ LC/MS grade), acetonitrile (MeCN), methanol (MeOH), and other common solvents (HPLC grade) were purchased from Fisher Scientific Co. (Fair Lawn, NJ, USA). Blank human plasma (K2EDTA added as anticoagulant) was obtained from Biological Specialty Co (Colmar, PA, USA); 10% MeCN 0.5% FA solution was prepared by adding 5 mL MeCN and 250 μL FA to 45 mL water (HPLC grade). Mobile phase A (20 mM NH4FA, 0.14%TFA) was prepared by adding 1.262 g NH4 FA and 1.4 mL TFA in 1 L water. Mobile phase B (0.1%TFA) was prepared by mixing 200 mL MeCN, 800 mL methanol and 1 mL TFA in 1 L glass bottle.
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