For alkaline phosphatase (ALP) staining, cells were cultured for 7 days in OΜ medium, fixed in 4% paraformaldehyde and stained with an ALP staining kit according to the manufacturer’s protocol (C3206, Beyotime, China). The formation of mineralized nodules of cells cultured for 21 days in OΜ medium were evaluated by Alizarin Red S staining (ALIR-10001, Cyagen, Taicang, China) and the lipid droplets of cells cultured in AΜ medium for 19 days were stained with Oil-Red O (OILR-10001, Cyagen, China).
Oilr 10001
Manufactured by Cyagen
Sourced in China
OILR-10001 is a laboratory equipment designed for oil analysis. It provides precise measurements and data collection capabilities for various oil samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using oilr 10001
1
Osteogenic and Adipogenic Differentiation Assessment
2
Senescence and Lipid Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fresh tissue was embedded with a frozen section embedding agent and sliced at −20 °C. SA-β-gal staining was performed using the senescence-associated β-galactosidase staining kit (C0602, Beyotime Co., Shanghai, China). Oil Red O staining was performed using Oil Red O (OILR-10001, Cyagen Biosciences, Guangzhou, China). After staining, the nuclei were re-stained and then sealed for observation.
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