Ibafect reagent

Dulbecco Modified Eagle’s Medium (DMEM), RPMI-1640, fetal bovine serum (FBS), penicillin, streptomycin, and phosphate-buffered saline (PBS) were provided from Gibco, Thermo Fisher Scientific (Carlsbad, CA, USA); ECL System and Bradford assay reagents were from Bio-Rad (München, Germany); protease inhibitor cocktail tablets (4693116001) from Roche Diagnostics (Grenoble, France); bovine serum albumin (BSA) (9048-46-8), formaldehyde solution 37% (F15587), methanol (34860), trizma base (1503), glycine (G8898), acrylamide/bis-acrylamide 30% solution (A3699) from Sigma-Aldrich (St. Louis, MI, USA); ProLongTM Gold Antifade Mountant with DAPI (P36935) from Thermo Fisher Scientific; IBAfect reagent (7-2005-050) from IBA Lifesciences (Goettingen, Germany); MitoTracker Red CMXRos (M7512) from Invitrogen (Carlsbad, CA, USA); MEK inhibitor PD098059 [2-(2′-amino-3′-methoxyphenyl)-oxanaphthalen-4-one] (10006726) and PI3K inhibitor LY294002 [2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4- one] (70920) from Cayman Chemical (Ann Arbor, MI, USA).

4′,6-diamidino-2-phenylindole (DAPI) (D9542, Sigma-Aldrich); SDS-PAGE reagents (Bio-Rad Laboratories, Hercules, CA, USA); PD98059 [2-(2′-amino-3′-methoxyphenyl)-oxanaphthalen-4-one] (513,000, Calbiochem, La Jolla, CA, USA); AG1478 [N-(3-chlorophenyl)−6,7-dimethoxy-4-quinazolinamine], (T4182, Sigma-Aldrich); puromycin, streptomycin and penicillin, and bovine serum albumin (BSA) (Sigma-Aldrich); fetal bovine serum (FBS) (GIBCO, Karlsruhe, Germany); LysoTracker (L7528, Thermo Fisher Scientific, Fremont, CA, USA), phalloidin (P1951, Sigma-Aldrich); lectin from Triticum vulgaris (wheat) FITC conjugate (L4895, Sigma-Aldrich); IBAfect reagent (7-2005-050, IBA Lifesciences, Goettingen, Germany); 4-methylumbelliferyl-N-acetyl-α-D-glucosaminide (474,500, Calbiochem); Trizol reagent (15596026, Invitrogen, Carlsbad, CA, USA).