All isolated metabolites were carefully screened by analytical TLC plates (Merck, Germany) under ultraviolet-visible detector with λ 254 nm. Separations were performed by silica gel (200–300 mesh, Yantai Chemical Co., Ltd., China), ODS (YMC Co., Japan), and Sephadex LH-20 (Mitsubishi Chemical Co., Japan) column chromatography. Generally, all target products were purified through an analytical HPLC (Shimadzu LC-10AVP Plus) with a RP-C18 column (5 μm, 10 × 250 mm, Welchrom®, China) and a CHIRALPAK®IC column (5 μm, 10 × 250 mm, Daicel, China). HRESIMS data were acquired by a Thermo Scientific LTQ-Orbitrap XL apparatus. IR spectra were measured on a Bruker Vertex 70 spectrophotometer using KBr discs. UV spectra were recorded using a Varian Cary 50 instrument. Circular dichroism chiroptical spectra were performed using a JASCO J-1700 spectrometer. The 1H (400 MHz) and 13C (100 MHz) NMR spectra were obtained using a Bruker AM-600 spectrometer with tetramethylsilane (TMS) as an internal standard while the chemical shifts were characterized to solvent peaks (CD3OD, δH 3.31 ppm; δC 49.15 ppm).
Cary 50 instrument
Manufactured by Agilent Technologies
The Cary 50 is a UV-Vis spectrophotometer manufactured by Agilent Technologies. It is designed to measure the absorbance or transmittance of light through a sample across the ultraviolet and visible light spectrum.
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Lab products found in correlation
Ltq orbitrap xl apparatus, by Thermo Fisher Scientific (1 mentions)
Am 600 spectrometer, by Bruker (1 mentions)
Chiralpak ic column, by Daicel (1 mentions)
341 polarimeter, by PerkinElmer (1 mentions)
Lc ltq orbitrap xl spectrometer, by Thermo Fisher Scientific (1 mentions)
J 810 spectrometer, by Jasco (1 mentions)
Apex duo diffractometer, by Bruker (1 mentions)
Am 400 spectrometer, by Bruker (1 mentions)
Sephadex lh 20, by Solarbio (1 mentions)
2 protocols using cary 50 instrument
1
Isolation and Characterization of Natural Metabolites
2
Phytochemical Characterization of Natural Compounds
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TLC was accomplished by silica gel 60 F254+365 (Qingdao Ocean Chemical Co., Ltd., China). Silica gel (80–120 and 200–300 mesh; Qingdao Ocean Chemical Co., Ltd., China), RP‐C18 silica gel (spherical, 20–45 μm, Santai Technologies, Inc., China), and Sephadex LH‐20 (Beijing Solarbio Science & Technology Co., Ltd., China) were utilized for column chromatography. HPLC was completed on an Essentia Prep LC‐16P with a UV detector via an RP‐C18 column (5 μm, 10×250 mm, YMC‐Pack, ODS‐A). Thermo Fisher LC‐LTQ‐Orbitrap XL spectrometer was used to collect the HRESIMS data. Bruker AM‐400 spectrometer was employed to obtain NMR data as well as taking the solvent peaks (CH3OH‐d4: δH 3.31, δC 49.10; DMSO‐d6: δH 2.50, δC 39.51) as internal references of the 1H and 13C NMR chemical shifts. JASCO J‐810 spectrometer was taken to carry out the ECD spectra measurement. Infrared spectra data were collected via Bruker Vertex 70 equipment. Ultraviolet spectra were analyzed by a Varian Cary 50 instrument. The single‐crystal X‐ray diffraction data were gathered by a Bruker APEX DUO diffractometer under the graphite‐monochromated Cu Kα radiation. The optical rotation measurement was fulfilled by a Perkin‐Elmer 341 polarimeter.
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