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Pe bdca 3

Manufactured by Miltenyi Biotec
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PE-BDCA-3 is a fluorochrome-conjugated antibody that targets the BDCA-3 antigen. BDCA-3 is a cell surface marker expressed on a subset of dendritic cells. The PE-conjugated version of this antibody can be used for the identification and analysis of BDCA-3-positive cells in flow cytometry applications.

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Lab products found in correlation

Fc500 mpl, by Beckman Coulter (2 mentions) Pe bdca 1, by Miltenyi Biotec (2 mentions) Pe bdca 2, by Miltenyi Biotec (2 mentions) Cd19 fitc, by Beckman Coulter (1 mentions) Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions) Cxp software, by Beckman Coulter (1 mentions)

2 protocols using pe bdca 3

1

Immunophenotyping of Leukocyte Subsets

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The following monoclonal antibodies were used in the present study: FITC-CD4/PE-CD8/PerCP-CD3, FITC-CD3/PE-anti-CD 16+56, FITC-CD19, and PECy5-CD14 (clone RMO52) were purchased from Beckman Coulter, CA, USA. FITC-HLA-DR (clone Immu-357) was purchased from BD Pharmingen, CA, USA. PE-BDCA-1, PE-BDCA-2, and PE-BDCA-3 were purchased from Miltenyi, Germany. Intracellular staining for PE- IL-17 (clone eBio64CAP17, eBioscience, USA) and PE-IFN-γ (clone 4SB3, eBioscience, USA) were executed with Cytofix/CytopermFixation/Permeabilization Kit (BD Pharmingen, CA, USA) according to the manufacturer's instructions. Flow cytometry was performed on the Beckman Coulter FC 500 MPL with CXP software.
Diao H., Cui G., Wei Y., Chen J., Zuo J., Cao H., Chen Y., Yao H., Tian Z, & Li L. (2014). Severe H7N9 Infection Is Associated with Decreased Antigen-Presenting Capacity of CD14+ Cells. PLoS ONE, 9(3), e92823.
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2

Immune Cell Profiling by Flow Cytometry

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The following monoclonal antibodies were used in the present study: fluorescein isothiocyanate (FITC)-anti-CD4 and phycoerythrin (PE)-anti-CD8/PerCP-anti-CD3, and PE-anti-iNKT (BD Pharmingen, CA, USA); FITC-anti-CD4, PE-anti-CD25, and FITC-anti-CD3/PE-anti-CD (16+56) (Invitrogen). PE-BDCA-1, PE-BDCA-2 and PE-BDCA-3 (Miltenyi, Germany). Cells were stained with the appropriate antibodies and incubated in 4°C for 30 min according to standard procedures. Flow cytometry was performed on the Beckman Coulter FC500 MPL (Beckman Coulter, Brea, CA, USA), and CXP software (Beckman coulter, CA, USA) was used for analysis.
Zheng Q., Cui G., Chen J., Gao H., Wei Y., Uede T., Chen Z, & Diao H. (2015). Regular Exercise Enhances the Immune Response Against Microbial Antigens Through Up-Regulation of Toll-like Receptor Signaling Pathways. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 37(2).
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