Rabbit anti camkii

Manufactured by Cell Signaling Technology

Rabbit anti-CaMKII is a primary antibody that recognizes the calcium/calmodulin-dependent protein kinase II (CaMKII) enzyme. CaMKII is a crucial regulator of cellular signaling pathways and plays a key role in various physiological processes. This antibody can be used to detect and quantify CaMKII levels in biological samples through techniques such as Western blotting, immunohistochemistry, and immunoprecipitation.

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CaMKII (32 citations) Anti-CaMKII (15 citations)

4 protocols using rabbit anti camkii

Western Blot Analysis of Apoptosis Markers

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Cells were lysed with RIPA buffer, and protein concentration was determined by BCA assay (Pierce, Waltham, MA). Protein lysates were separated by SDS–PAGE, transferred to nitrocellulose membranes (Bio-Rad), blocked with 5% milk/TBS, and probed with antibodies in 4°C overnight. The following antibodies were used for Western blot: mouse anti-CRT (Enzo Life Sciences, Farmingdale, NY), mouse anti-GAPDH (Fitzgerald, Acton, MA), rabbit anti-caspase 3 (Cell Signaling Technology, Danvers, MA), rabbit anti-caspase 7 (Cell Signaling Technology, Danvers, MA), rabbit anti-BAX antibody (abcam, Cambridge, MA), rabbit anti-PARP (Cell Signaling Technology, Danvers, MA), mouse anti-PARP C2-10 (Trevigen, Gaithersburg, MD), mouse anti-p53 (Santa Cruz, Dallas, TX), rabbit anti-CaMKII and anti-phospho CaMKII (Cell Signaling Technology, Danvers, MA).

Han A., Li C., Zahed T., Wong M., Smith I., Hoedel K., Green D, & Boiko A.D. (2019). Calreticulin is a Critical Cell Survival Factor in Malignant Neoplasms. PLoS Biology, 17(9), e3000402.

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Antibody Validation for Neuroscience Research

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The rabbit polyclonal antibody against GluN2A phospho-S1459 was custom-made and has been validated previously (Mota Vieira et al., 2020 (link)). The following antibodies were purchased from commercial sources: mouse anti-β-actin (Cat# sc-47778, Santa Cruz Biotechnology), rabbit anti-CaMKII (Cat# 4436, Cell Signaling Technology), chicken anti-GFP (Cat# GFP-1020, Aves Labs); rabbit anti-GFP (Cat# 504302-AP, Proteintech), rabbit anti-GluN2A (Cat# 04-901, Millipore), mouse anti-GFP (Cat# ab1218, Abcam), mouse anti-GST (Cat# 66001-2-Ig, Proteintech), mouse anti-myc (Cat# sc-40, Santa Cruz Biotechnology), rabbit anti-SNX27 (Cat# 16329-1-AP, Proteintech), rabbit anti-VPS26 (Cat# ab181352, Abcam), rabbit anti-VPS35 (Cat# 10236-1-AP, Proteintech) and chicken anti-MAP2 (cat# ab92434, Abcam). The polyclonal rabbit antibody against GFP (JH4030) was a gift from Dr. Richard Huganir (Anggono et al., 2011 (link)). Alexa-conjugated and HRP-conjugated secondary antibodies were purchased from Thermo Scientific and GE Healthcare, respectively.

Hilary Yong X.L., Zhang L., Yang L., Chen X., Tan J.Z., Yu X., Chandra M., Livingstone E., Widagdo J., Vieira M.M., Roche K.W., Lynch J.W., Keramidas A., Collins B.M, & Anggono V. (2021). Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit. Cell reports, 36(1), 109338.

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Antibody Validation for Neuroscience Research

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Western Blot Assay for Synaptic Proteins

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Protein concentration was determined by the BCA protein assay kit. Proteins (20 μg) were resolved in SDS-polyacrylamide gels and electrotransferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA). Nonspecific binding sites on the blotting membrane were blocked with 5% non-fat dry milk for 2 h and incubated at 4°C overnight with mouse anti-NR2B (1∶1000, Abcam), rabbit anti-SAP102 (1∶500, Santa Cruz), rabbit anti-CaMKII (1∶1000, Cell Signaling), rabbit anti-SynGAP (1∶1000, Abcam), rabbit anti-pERK1/2 (1∶2000, Cell Signaling), rabbit anti-pCREB (1∶1000, Cell Signaling Technology) and rabbit anti-BDNF (1∶1000, Sigma). The proteins were detected with anti-rabbit or anti-mouse secondary antibody for 2 h at room temperature. Then, the proteins were visualized with BeyoECL Plus reagents and captured by an enhanced chemiluminescence system (ECL kit, Pierce Biotechnology, Inc.).

Huo X.L., Min J.J., Pan C.Y., Zhao C.C., Pan L.L., Gui F.F., Jin L, & Wang X.T. (2014). Efficacy of Lovastatin on Learning and Memory Deficits Caused by Chronic Intermittent Hypoxia-Hypercapnia: Through Regulation of NR2B-Containing NMDA Receptor-ERK Pathway. PLoS ONE, 9(4), e94278.

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