Il 17a

Manufactured by Cell Signaling Technology

Sourced in United States

IL-17A is a cytokine that plays a role in the inflammatory response. It is a product of Cell Signaling Technology's lab equipment line.

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Lab products found in correlation

3 protocols using il 17a

Comprehensive Immune Cell Profiling

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Cells were stained with the following antibodies: 0.1 µl/100 μl Fixable Viability Dye (eFluor 520, eFluor 780), 0.25 µg/100 μl CD4 (Brilliant violet 711, clone: GK1.5), 0.5 µg/100 μl RORγt (APC, clone: AFKJS-9), 0.4 µg/100 μl T-bet (PE-cyanine7, clone: 4B10), 0.5 µg/100 μl IL-17A (eFluor 450, clone: eBio17B7), 0.25 µg/100 μl GM-CSF (PE, clone: MP1-22E9), 0.25 µg/100 μl IFNγ (FITC, clone: XMG1.2), 0.5 µg/100 μl CD25 (PE-Cy7, clone: PC61), 0.06 µg/100 μl Foxp3 (eFluor 450, FITC, clone: FJK-16s), 0.5 µg/100 μl CD8α (PE, clone: 53-6.7), 5 µg/100 μl IL-23R (PE, BV711, clone: O78-1208), 0.5 µg/100 μl CCR6 (BV605, clone: 29-2L17), 1 µg/100 μl CD29 (PE-Cy7, clone: HMβ1-1), 2 µl/100 μl Malt1 (#2494 Cell Signaling Technology, Danvers, MA, USA), 5 µg/ml Alexa Fluor 488 goat anti-rabbit IgG (H + L) (A11008, Fisher Scientific, Thermo Fisher Scientific, PA, USA), 2 µl/100 μl pStat3 Y705 (#4324, Alexa Fluor 647, clone: D3A7), 0.25 µg/100 μl CD44 (APC, clone: IM7), 0.5 µg/100 μl CD62L (PE, clone: MEL-14), 0.5 µg/100 μl CD69 (PerCP-Cy5.5, clone: H1.2F3), 0.25 µg/100 μl Ki-67 (FITC, clone: SolA15), 1 µl/100 μl NF-κB p65 (#4764, clone: C22B4), 1 µl/100 μl RelB (#4922, clone: C1E4), 5 µg/ml GFP (A21311, Alexa Fluor 488), 0.125 µg/100 μl CD90.1 (PE-Cy7, clone: HIS51).

Cho J.J., Xu Z., Parthasarathy U., Drashansky T.T., Helm E.Y., Zuniga A.N., Lorentsen K.J., Mansouri S., Cho J.Y., Edelmann M.J., Duong D.M., Gehring T., Seeholzer T., Krappmann D., Uddin M.N., Califano D., Wang R.L., Jin L., Li H., Lv D., Zhou D., Zhou L, & Avram D. (2019). Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation. Nature Communications, 10, 701.

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Investigating IL-17A Signaling Modulation

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Dimethylsulfoxide (DMSO) (Cat: D2650), 4-PBA (Cat: P21005) was purchased from Sigma (St.Louis, MO, USA). Recombinant Mouse IL-17A (rmIL-17A) (Cat: 7956-ML) and IL-17ANab (Cat: MAB421) were purchased from R&D Systems (Minneapolis,MN,USA). MCC950 (Cat: S7809) was purchased from Selleck Chemicals (Houston, TX, USA), TM (Cat: ab120296) and primary antibodies against F4/80 (Cat: ab16911), ATF-4 (Cat: ab216839), GRP78 (Cat: ab109659), CHOP (Cat: ab10444), IL-1β (Cat: ab234437), β-actin (Cat: ab179467) were purchased from Abcam (Cambridge, MA, USA), primary antibodies against TXNIP (Cat:14715S), NLRP3 (Cat: 15101S), IL-17A (Cat: 13838S) were purchased from Cell Signaling Technology (Ozyme, SaintQuentin-en-Yvelines, France). Dulbecco’s modified Eagle’s medium (DMEM) (Cat: 10,567,014), Minimum Essential Medium (MEM) α (Cat: 32,561,037), fetal bovine serum (FBS) (Cat:10,099,141) and penicillin–streptomycin (Cat:10,378,016) were obtained from Thermo Scientific (Logan, UT, USA).

Wang Y., Gao S., Gao S., Li N., Xie B, & Shen X. (2021). Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy. Cell & Bioscience, 11, 82.

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Psoriatic Keratinocyte Culture Assay

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Skin biopsy specimens (6 mm) were obtained from both the lesional skin of patients with psoriasis and the skin of individuals without psoriasis under an Oregon Health & Science University Institutional Review Board-approved protocol. The epidermis was trimmed off and minced into 1×1 mm² skin islets. The skin islets were planted on collagen-coated plates as described[20 (link)]. Keratinocytes from the outgrowth were trypsinized and plated in 12-well plates for the assay. The keratinocyte cultures were switched to CnT-07 keratinocyte medium (Zen Bio) that contains 10 ng/ml EGF or Epilife medium with or without human keratinocyte growth supplement (Invitrogen) that contains 0.2 ng/ml EGF for 3 days before cytokine treatments, which included 10 ng/ml TNFα (Prospec), 20 ng/ml IL-17A (Cell Signaling), 20 ng/ml IL-22 (Cell Signaling), 10 ng/ml interferonγ (Prospec), 20 ng/ml IL-17F (Cell Signaling), and 10 ng/ml growth factors EGF (Millipore), 10 ng/ml transforming growth factor alpha (TGFα; Biolegend), amphiregulin (AREG; R&D Systems), and 10 ng/ml HB-EGF (R&D Systems) as indicated. The EGFR inhibitors erolotinib, gefitinib, and lapatinib were purchased from SelleckChem. The toll like receptor (TLR) agonist peptidoglycan was purchased from Sigma.

Ehst B., Wang Z., Leitenberger J., McClanahan D., De La Torre R., Sawka E., Ortega-Loayza A.G., Strunck J., Greiling T., Simpson E, & Liu Y. (2020). Synergistic induction of IL-23 by TNFα, IL-17A, and EGF in keratinocytes. Cytokine, 138, 155357.

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