Cell culture consumables

All chemicals, media, and supplements for cell culture and ALI exposure were purchased from Gibco/Thermo Fisher Scientific GmBH (Dreieich, Germany) and Roth (Karlsruhe, Germany), except for KGM, which was acquired from Lonza (Basel, Switzerland). The PAN-based CF Tenax^®-J UMS40 was purchased from Teijin Carbon Europe GmbH (Wuppertal, Germany). Cell culture consumables were obtained from Greiner (Kremsmünster, Germany), and Transwell^® plates and inserts were produced and purchased from Corning (Amsterdam, The Netherlands). LDH assay kits were bought from Roche (Mannheim, Germany). Triton X-100 was purchased from Roth (Karlsruhe, Germany). Primers for RT-qPCR were synthesized by Eurofins (Ebersberg, Germany). PCR reagents were acquired from Macherey-Nagel (Dueren, Germany), Applied Biosystems (Foster City, CA, USA), Teknova (Hollister, OH, USA), Fluidigm (San Francisco, CA, USA), Bio-Rad (Munich, Germany), and New England Biolabs (Frankfurt am Main, Germany). IL-8 ELISA kits were obtained from Invitrogen (Waltham, MA. USA). LPS and Menadione were purchased from Sigma-Aldrich (Steinheim, Germany).

Cell culture media and additives were from Biochrom, Thermo Fisher Scientific or Invitrogen. Cell culture consumables were from Greiner Bio-One. Chemicals, reagents western blot equipment and further consumables were obtained from Carl Roth, Sigma Aldrich, Sarstedt or Thermo Fisher. Primer and peptides were synthesized by Iba or JPT. Restriction enzymes, ligases and phosphatases were from NEB. DNA purification kits were from Machery & Nagel. Anti-receptor antibodies were from Biolegend and RnD systems. Secondary antibodies and conjugates were from Jackson Immuno Research. Agonists and antagonists were obtained from Merck, Peprotech, Perkin Elmer or Sigma Aldrich.