Cd40 clone 5c3

Manufactured by BioLegend

CD40 (clone 5C3) is a mouse monoclonal antibody that recognizes the CD40 antigen. CD40 is a member of the tumor necrosis factor receptor superfamily and is expressed on the surface of various cell types, including B cells, dendritic cells, and endothelial cells. The antibody can be used to detect and study the expression of CD40 in research applications.

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Lab products found in correlation

Cd16 clone 3g8, by BioLegend (1 mentions) Lsr fortessa cytometer, by BD (1 mentions) Cd80 clone 2d10, by BioLegend (1 mentions) Hla dr clone l243, by BioLegend (1 mentions) Cd8 clone rpa t8, by BioLegend (1 mentions) Cd4 clone rpa t4, by BioLegend (1 mentions) Foxp3 clone 206d, by BioLegend (1 mentions) Cd11c clone b ly6, by BD (1 mentions)

Spelling variants of this product

Anti-human CD40 (clone 5C3 (3 citations) CD40 (5C3) (2 citations)

2 protocols using cd40 clone 5c3

Immunophenotyping of COVID-19 Patients' Leukocytes

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Whole blood samples from COVID-19 patients were diluted with Tyrode’s/HEPES buffer at 1:5. Cells were stained with anti–human CD66b (clone G10F5, BioLegend), CD16 (clone 3G8, BioLegend), and CD40 (clone 5C3, BioLegend); platelet marker anti–human CD41 (clone HIP8, BioLegend); and platelet activation marker anti–human CD62P (clone AK4, BioLegend) for 10 minutes at RT in the dark. Cells were fixed with 1% paraformaldehyde for 10 minutes and then acquired by FACSCanto.

Morrissey S.M., Geller A.E., Hu X., Tieri D., Ding C., Klaes C.K., Cooke E.A., Woeste M.R., Martin Z.C., Chen O., Bush S.E., Zhang H.G., Cavallazzi R., Clifford S.P., Chen J., Ghare S., Barve S.S., Cai L., Kong M., Rouchka E.C., McLeish K.R., Uriarte S.M., Watson C.T., Huang J, & Yan J. (2021). A specific low-density neutrophil population correlates with hypercoagulation and disease severity in hospitalized COVID-19 patients. JCI Insight, 6(9), e148435.

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Comprehensive Immune Profiling by Flow Cytometry

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Antibodies for CD4 (clone RPA-T4, Biolegend), CD8 (clone RPA-T8, Biolegend), CD11c (clone Bly6, BD Biosciences), CD40 (clone 5C3, Biolegend), CD80 (clone 2D10, Biolegend), CD86 (clone BU63, Biolegend), HLA-DR (clone L243, Biolegend), Galectin 9 (clone 9M1-3, Biolegend), PDL1 (clone), CD25 (clone BC96, Biolegend), FOXP3 (clone 206D, Biolegend), ILT3 (clone ZM4.1, Biolegend), ILT4 (clone 42D1, Biolegend), PD1 (clone EH12, BD Biosciences), Eomes (clone WD1928, eBioscience), IDO (clone eyedio, eBioscience) were obtained, and cells were stained according to manufacturer-recommended protocols. Events were collected on an LSRFortessa cytometer (BD), and data were analyzed in FlowJo (Treestar). Representative gating strategies are presented in Supplemental Figure 1.

Brusko M.A., Stewart J.M., Posgai A.L., Wasserfall C.H., Atkinson M.A., Brusko T.M, & Keselowsky B.G. (2020). Immunomodulatory Dual-Sized Microparticle System Conditions Human Antigen Presenting Cells Into a Tolerogenic Phenotype In Vitro and Inhibits Type 1 Diabetes-Specific Autoreactive T Cell Responses. Frontiers in Immunology, 11, 574447.

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