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683 infrared spectrometer

Manufactured by PerkinElmer
Sourced in Japan

The 683 infrared spectrometer is a laboratory instrument designed to measure the infrared spectrum of a sample. It is capable of detecting and analyzing the absorption or transmission of infrared light by various materials.

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4 protocols using 683 infrared spectrometer

1

Comprehensive Analytical Characterization

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An electrospray ionization source (ESI)-equipped Q-Exactive Mass spectrometer (Thermo Fisher Scientific Corporation, Waltham, MA, USA) was used to analyze the HR-ESI-MS data. A Shimadzu UV-260 spectrometer (Shimadzu Corporation, Tokyo, Japan) and a Perkin-Elmer 683 infrared spectrometer (PerkinElmer, Inc., Waltham, MA, USA) were used to obtain the UV and IR spectra, respectively. A JASCO P-200 polarimeter (JASCO Corporation, Tokyo, Japan) with a 5 cm cell was applied to measure the optical rotation value. The NMR spectra with TMS as the internal standard were taken on a Brucker Avance III 600 FT NMR spectrometer (Bruker Corporation, Billerica, MA, USA).
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2

Spectroscopic Characterization of Compounds

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Optical rotations were measured on a Perkin-Elmer 241 polarimeter. CD spectra were obtained on a JASCO J-815 spectropolarimeter. UV spectra were obtained on a Shimadzu UV-160 spectrophotometer. IR spectra were recorded on a Perkin-Elmer 683 infrared spectrometer with KBr disks. HR-ESIMS were recorded on a Bruker Daltonics on a Micromass LCT mass spectrometer. NMR spectra were recorded on an ECZ400S spectrometer, with TMS as the internal standard. silica gel (200–300 mesh) for column chromatography (CC) and silica gel GF254 for preparative TLC were obtained from Qingdao Marine Chemical Factory, Qingdao, People's Republic of China. Preparative HPTLC (high performance thin layer chromatography) was conducted on Merck precoated silica gel 60 F254 plates, and detected under UV light.
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3

Spectroscopic Characterization of Natural Products

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An electrospray ionization source (ESI)-equipped Q-Exactive Mass spectrometer (Thermo Fisher Scientific Corporation, Waltham, MA, USA) was used to analyse the HR-ESI-MS data. A Shimadzu UV-260 spectrometer (Shimadzu Corporation, Tokyo, Japan) and a Perkin–Elmer 683 infrared spectrometer (PerkinElmer, Inc., Waltham, MA, USA) were used to obtain the UV and IR spectra, respectively. A JASCO P-200 polarimeter (JASCO Corporation, Tokyo, Japan) with a 5 cm cell was applied to measure the optical rotation value. The NMR spectra with TMS as the internal standard were taken on a Brucker Avance III 600 FT NMR spectrometer (Bruker Corporation, Billerica, MA, USA).
Column chromatography was performed with silica gel (Yantai Chemical Industry Research Institute, Shandong, China), Cosmosil 75 C18-MS-II (75 μm, Nacalai Tesqye corporation, Kyoto, Japan), and Spehadex LH-20 (GE Healthcare, Danderyd, Sweden). Semi-preparative HPLC was conducted on an Aglient HPLC (Agilent Technologies Inc., Santa Clara, CA, USA) system equipped with a diode array detector via a preparative Cosmosil ODS column. The HR-ESI-MS spectra were measured using a thermo Q-Exactive Mass spectrometer (Thermo Fisher Scientific Corporation).
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4

Comprehensive Analytical Characterization of Compounds

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An electrospray ionization source (ESI)-equipped Q-Exactive mass spectrometer (Thermo Fisher Scientific Corporation, Waltham, MA, USA) was used to analyze the HR-ESI-MS data. A Shimadzu UV-260 spectrometer (Shimadzu Corporation, Tokyo, Japan) and a Perkin-Elmer 683 infrared spectrometer (PerkinElmer, Inc., Waltham, MA, USA) were used to obtain the UV and IR spectra, respectively. A JASCO P-200 polarimeter (JASCO Corporation, Tokyo, Japan) with a 5 cm cell was applied to measure the optical rotation value. The NMR spectra with TMS as the internal standard were taken on a Brucker Avance III 600 FT NMR spectrometer (Bruker Corporation, Billerica, MA, USA).
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