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Mouse cxcl10 quantikine elisa kit

Manufactured by R&D Systems

The Mouse CXCL10 Quantikine ELISA kit is a solid-phase enzyme-linked immunosorbent assay (ELISA) designed to measure mouse CXCL10 levels in cell culture supernates, serum, and plasma. It utilizes the quantitative sandwich enzyme immunoassay technique.

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2 protocols using mouse cxcl10 quantikine elisa kit

1

Measurement of Interferon-β and CXCL10

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Tumor tissues were homogenized in PBS with protease inhibitor followed by addition of Triton X-100. Cell culture supernatants were obtained from isolated CD11c+ cells after 48 hr-incubation with fresh GM-CSF. The concentration of IFN-β and CXCL10 was measured with VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) and mouse CXCL10 Quantikine ELISA kit (R&D) in accordance with the manufacturer’s instructions, respectively.
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2

Radiation-Induced Tumor Cytokine Analysis

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Tumor tissues were excised on day 3 after radiation and homogenized in PBS with protease inhibitor. After homogenization, Triton X-100 was added to obtain lysates. Cell culture supernatants were obtained from isolated CD11c+ cells after 48h-incubation with fresh GM-CSF. The concentration of IFN-β and CXCL10 was measured with VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) and mouse CXCL10 Quantikine ELISA kit (R&D) in accordance with the manufacturer's instructions, respectively.
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