The apoptotic rate of U251 and DBTRG cells was detected using an Annexin V, 633 Apoptosis Detection Kit (Dojindo Molecular Technologies), following the kit instructions. U251 and DBTRG cells were seeded into 6-well plates (5 × 105 cells/well) and cultured for 12 h. Next, U251 and DBTRG cells were incubated with Annexin V, followed by propidium iodide (PI) buffer for 15 min at 25 °C in a dark room. Subsequently, apoptotic cells were quantified using a NovoCyte 1040 flow cytometer (ACEA Biosciences, Inc., Zhejiang, China).
Novocyte 1040 flow cytometer
Manufactured by Agilent Technologies
Sourced in China
The NovoCyte 1040 is a flow cytometer designed for cell analysis. It is capable of detecting and analyzing cells or particles in a fluid sample.
Automatically generated - may contain errors
3 protocols using novocyte 1040 flow cytometer
1
Quantification of Apoptosis in U251 and DBTRG Cells
2
Arsenic trioxide induces apoptosis in T24 cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T24 cells were seeded into 96-well plates (2 × 104 cells/well) and cultured for 12 h. Next, T24 cells were incubated with As2O3 (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) at 0, 10, and 20 μmol/L for 6 h. After washing, T24 cells were incubated with 10% CCK-8 (Dojindo Molecular Technologies, Inc., Minato-ku, Tokyo, Japan) and optical density measured using a xMark Microporous Plate Absorption Spectrophotometer (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
The apoptotic rate of T24 cells was detected using an Annexin V, 633 Apoptosis Detection Kit (Dojindo Molecular Technologies), following the kit instructions. T24 cells were seeded into 6-well plates (5 × 105 cells/well) and cultured for 12 h. Next, cells were incubated with As2O3 (Sigma-Aldrich) at 20 μmol/L for 6 h, then incubated with Annexin V, followed by propidium iodide (PI) buffer for 15 min at 25°C in a dark room. Subsequently, apoptotic cells were quantified using a NovoCyte 1040 flow cytometer (ACEA Biosciences, Inc., Zhejiang, China).
The apoptotic rate of T24 cells was detected using an Annexin V, 633 Apoptosis Detection Kit (Dojindo Molecular Technologies), following the kit instructions. T24 cells were seeded into 6-well plates (5 × 105 cells/well) and cultured for 12 h. Next, cells were incubated with As2O3 (Sigma-Aldrich) at 20 μmol/L for 6 h, then incubated with Annexin V, followed by propidium iodide (PI) buffer for 15 min at 25°C in a dark room. Subsequently, apoptotic cells were quantified using a NovoCyte 1040 flow cytometer (ACEA Biosciences, Inc., Zhejiang, China).
3
Quantifying Apoptosis in FaDu and Cal27 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The apoptosis rate of Fadu and Cal27 cells was detected using an Annexin V 633 Apoptosis Detection Kit (Dojindo Molecular Technologies) following the kit instructions. The Fadu and Cal27 cells were seeded into 6-well plates (5 × 105 cells/well) and cultured for 12 h, and then they were incubated with Annexin V, followed by propidium iodide (PI) buffer for 15 min at 25 °C in a dark room. Subsequently, apoptotic cells were quantified using a NovoCyte 1040 flow cytometer (ACEA Biosciences, Inc., Zhejiang, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!