Penta his antibody

Manufactured by Thermo Fisher Scientific

The Penta-His Antibody is a laboratory reagent used for the detection and purification of proteins that have been engineered to contain a histidine-tag (His-tag). The antibody specifically binds to the His-tag, allowing for the identification and isolation of the target protein from complex mixtures.

Automatically generated - may contain errors

Lab products found in correlation

Human serum albumin (hsa), by Merck Group (2 mentions) Allegra 25r centrifuge, by Beckman Coulter (2 mentions) N octyl β d glucopyranoside og detergent, by Anatrace (2 mentions) Akta purifier 10, by GE Healthcare (2 mentions) Glucose oxidase from aspergillus niger, by Merck Group (1 mentions)

2 protocols using penta his antibody

Protein Purification Using Chromatographic Columns

Check if the same lab product or an alternative is used in the 5 most similar protocols

For protein purification,
chromatographic
columns and an AKTA Purifier 10 from GE Healthcare (Pittsburgh, PA)
as well as rotors and an Allegra 25R centrifuge from Beckman Coulter
(Indianapolis, IN) were employed. The Penta-His Antibody from Thermo
Scientific (Waltham, MA) was used for Western blot detection. Glucose
oxidase from Aspergillus niger (160 kDa) and human
serum albumin (66.5 kDa) were purchased from Sigma-Aldrich (St. Louis,
MO). The n-octyl β-d-glucopyranoside
(OG) detergent was from Anatrace (Maumee, OH). All other chemicals
and reagents were from Fisher Scientific (Waltham, MA).

Zhang X., Bayles K.W, & Luca S. (2017). Staphylococcus aureus CidC Is a Pyruvate:Menaquinone Oxidoreductase. Biochemistry, 56(36), 4819-4829.

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Protein Purification using AKTA Purifier

Check if the same lab product or an alternative is used in the 5 most similar protocols

For protein purification, chromatographic columns and an AKTA Purifier 10 from GE Healthcare (Pittsburgh, PA), as well as rotors and an Allegra 25R centrifuge from Beckman Coulter (Indianapolis, IN) were employed. The Penta-His Antibody from Thermo Scientific (Waltham, MA) was used for western blot detection. Glucose oxidase from Aspergillus niger (160 kDa) and human serum albumin (66.5 kDa) were purchased from Sigma Aldrich (St. Louis, MO). The n-octyl-β-D-glucopyranoside (OG) detergent was from Anatrace (Maumee, OH). All other chemicals and reagents were from Fisher Scientific (Waltham, MA).

Zhang X., Bayles K.W, & Luca S. (2017). Staphylococcus aureus CidC Is a Pyruvate:Menaquinone Oxidoreductase. Biochemistry, 56(36), 4819-4829.

+ Open protocol

+ Expand

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