A fluorescence detection system was designed to detect the PCR results. The detection system was made up of a CMOS camera (OV2710, OmniVision, Santa Clara, CA, USA), a blue LED (XLamp XP-E, Cree, Inc., Durham, NC, USA), and mirror units (U-FBW, Olympus, Tokyo, Japan). The results were displayed on a laptop. The mirror units contained a dichroic mirror, an excitation filter, and an emission filter. The blue LED was placed on the side of the filters and combined with a circular reflector to reduce the light decay. The reaction chips were illuminated by the blue LED, and the dominant wavelength of the LED was 465~485 nm. The CMOS camera captured the fluorescence signal through the emission filter as shown in the schematic diagram in Figure 7 a. A 3D-printed structure integrated the LED, the CMOS camera, and the mirror units together as shown in Figure 7 b. The blue LED, which had a power consumption of 3 W, was powered by the Li-ion battery. The CMOS camera was powered and driven by the laptop.
U fbw
Manufactured by Olympus
Sourced in Japan
The U-FBW is a microscope objective lens designed for brightfield and darkfield observation. It features a high numerical aperture for improved resolution and light-gathering capability. The lens is suitable for a range of sample types and biological applications.
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Lab products found in correlation
2 protocols using u fbw
1
Fluorescence Detection System for PCR
2
Microscopy Methods for Extracellular and Intracellular Polysaccharides
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To detect extracellular polysaccharides, a 100-μL aliquot of the cell culture, harvested from the main cultivation, was suspended in 20 μL India ink (Daiso Co., Ltd., Japan) and observed under an optical microscope (BX53: Olympus, Tokyo, Japan) at DIC (differential interference contrast) conditions with a shutter speed of 0.48 s. Alcian blue (#015-13805: Wako Co., Ltd., Osaka, Japan) staining was conducted for detecting acidic polysaccharides [[21] , [22] (link), [23] (link)]. PAS (#164-19705: Wako Co., Ltd.) staining was performed for detecting neutral polysaccharides [24 ]. Cells stained with Alcian blue or PAS were observed using BX53 (Olympus) at DIC conditions with a shutter speed of 0.48 s. Regarding intracellular lipid staining [25 (link)], a 1-mL aliquot of the cell culture was collected from the main cultivation and mixed with 20 μM of Nile red (#144-08811: Wako Co., Ltd.). Samples were observed for morphology using an optical photomicroscope at a high resolution with a Nomarski prism or using the fluorescence microscope, BX53/DP72, fitted with a special filter, U-FBW (Olympus) for excitation (460–495 nm) and emission (510 nm). The exposure time for photography was 0.2 s.
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