PBMCs isolated from patients with GCs or HVs were stained with the following antibodies: APC-Cy7 mouse anti-human CD8 (#555369, BD Biosciences), PE mouse anti-human PD-1 (# 563422, BD Biosciences), and PerCP-Cy5.5 mouse anti-human TIGIT (VSTM3, #372718, BioLegend). The molecular phenotypes of peripheral blood leucocytes were analysed immediately using flow cytometry (BD FACS Canto™ II). Samples were analysed using the FlowJo V10 (Tree Star) software.
Pe mouse anti human pd 1
Manufactured by BD
The PE mouse anti-human PD-1 is a fluorescently-labeled antibody that binds to the Programmed Cell Death 1 (PD-1) receptor on human cells. PD-1 is an important immune checkpoint protein that regulates T-cell activation and function. This antibody can be used for the identification and analysis of PD-1-expressing cells using flow cytometry or other immunoassays.
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Lab products found in correlation
2 protocols using pe mouse anti human pd 1
1
Phenotypic Analysis of Immune Cells
2
Characterization of CAR-T Cells and Tumor Cells
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Cancer cell lines and CAR-modified T cells were prepared according to the manufacturer’s instructions. The following monoclonal antibodies were obtained from BD Biosciences: phycoerythrin (PE)-mouse anti-human PD-1 (CD279), allophycocyanin (APC)-mouse anti-human PD-L1 (CD274), BB515-mouse anti-human IgG, V450-mouse anti-human CD62L, PE-mouse anti-human CTLA4 (CD152), APC-mouse anti-human CD27, APC-mouse anti-human CD28, PE-mouse anti-human TIM-3 (CD366), PE-mouse anti-human LAG-3 (CD223), and their IgG controls. After preparation, CAR-modified T cells and tumor cell lines were confirmed using the FACSCanto II flow cytometer and analyzed using CellQuest Pro (BD Biosciences) software.
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