Third instar wing discs were staged and fixed in 4% paraformaldehyde. Immuno-fluorescence was performed as described(Das et al., 2013 (link)). Antibodies used: anti-pRET[Y905], anti-pJnk, anti-pAkt, anti-SOX2, anti-slug, anti-NCadherin, anti-pEGFR[Y845], anti-pEGFR[Y1068], anti-pFGFR[Y653/654], anti-Rab5, anti-Rab7, anti-Rab9(Cell Signaling), anti-pSRC[Y418] (Invitrogen), anti-dpERK (SIGMA), plus anti-Actin,, anti-E-cadherin, anti-α-Catenin, anti-Rho1, anti-Syntaxin, anti-β-tubulin (Developmental Studies Hybridoma Bank), anti-Actin, anti-GAPDH antibodies (Santa Cruz Biotechnology), anti-Rac1 antibody (BD biosciences), anti-KIF5B(Abcam), anti-EGFR (Julia Cordero), anti-Arp3(William Theurkauf).
Anti syntaxin
Manufactured by Developmental Studies Hybridoma Bank
Anti-Syntaxin is a monoclonal antibody used for the detection and localization of the Syntaxin protein. Syntaxin is a member of the SNARE protein family and plays a crucial role in the docking and fusion of synaptic vesicles with the presynaptic membrane. The Anti-Syntaxin antibody can be utilized in various immunological techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the distribution and expression of Syntaxin in biological samples.
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Lab products found in correlation
Anti rab5, by Cell Signaling Technology (1 mentions)
Anti kif5b, by Abcam (1 mentions)
Anti dperk, by Merck Group (1 mentions)
Anti β tubulin, by Developmental Studies Hybridoma Bank (1 mentions)
Anti e cadherin, by Developmental Studies Hybridoma Bank (1 mentions)
Anti rab9, by Cell Signaling Technology (1 mentions)
Anti pfgfr, by Cell Signaling Technology (1 mentions)
Anti rac1 antibody, by BD (1 mentions)
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti actin, by Santa Cruz Biotechnology (1 mentions)
Anti p egfr, by Cell Signaling Technology (1 mentions)
Anti rab7, by Cell Signaling Technology (1 mentions)
Fluorescent secondary antibody, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti syntaxin
1
Immunofluorescence Analysis of Wing Disc
2
Synaptic Protein Expression in Drosophila
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Western blotting of whole adult head lysates (1 head/lane) was performed using standard laboratory procedures with anti-SYT1 (1:1,000, kindly provided by Noreen Reist) or anti-syntaxin (1:1,000, Developmental Studies Hybridoma Bank, Iowa City, IA). Visualization and quantification were done using a LI-COR Odyssey Imaging System (LI-COR Biosciences, Lincoln, MA). Immunostaining was performed on 3rd instar larvae at wandering stage larvae as described previously.9 (link) Rabbit myc antibody (1:1,000; Genetex) and anti-horseradish peroxidase (1:1,000; Jackson ImmunoResearch, West Grove, PA) were used for immunostaining. Confocal stacks of muscle 6 and 7 NMJs containing immunoreactive proteins were captured on a Zeiss Pascal Confocal with PASCAL software (Carl Zeiss MicroImaging, Inc.) using a 63 × numerical aperture 1.3 Plan Neofluar oil immersion lens (Carl Zeiss, Inc) and fluorescent secondary antibodies (Molecular Probes, Carlsbad, CA).
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