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Cleaved caspase 9

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Cleaved caspase-9 is a laboratory reagent used in the detection of apoptosis. It is a protein fragment generated by the activation of caspase-9, a key enzyme in the apoptotic pathway. This product can be used to measure the levels of cleaved caspase-9 as an indicator of cellular apoptosis.

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Lab products found in correlation

Cleaved caspase 3, by GeneTex (2 mentions) Cleaved parp, by GeneTex (1 mentions) Beta actin, by GeneTex (1 mentions) γ h2ax, by GeneTex (1 mentions) Image lab software, by Bio-Rad (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) Enhanced chemi luminescence (ecl), by PerkinElmer (1 mentions) Anhydrous ethanol, by Merck Group (1 mentions) Triphenylphosphine tpp, by Merck Group (1 mentions) Ammonium hydroxide nh3 h2o, by Merck Group (1 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions) N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions) Bcl 2, by GeneTex (1 mentions) Gapdh, by GeneTex (1 mentions) Sodium carbonate na2co3, by Sinopharm (1 mentions)

2 protocols using cleaved caspase 9

1

Signaling Pathway Analysis of DOK Cells

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First, 6 × 105 DOK
cells were seeded into 6 cm plates and incubated overnight. Following
the treatment of DOK cells with BAK, Stattic, or Wortmannin, cell
lysates were obtained and lysed in an RIPA buffer containing a proteinase
inhibitor. The protein lysates were loaded onto SDS-PAGE, and proteins
were transferred onto PVDF membranes and reacted with primary antibodies
followed by secondary antibodies (Jackson, USA). Finally, the signals
were amplified by ECL (Perkin Elmer, USA), imaged by ChemiDoc XRS+
System (BioRad, USA) and quantified by Image Lab software (BioRad,
USA). Primary antibodies against pSTAT3(Tyr705), STAT3, pAkt(Ser473),
Akt, cleaved caspase-3, cleaved caspase-9, cleaved caspase-7, cleaved
PARP, γH2AX, and beta-Actin (GeneTex, USA) were used for Western
blotting.
Wang Y.Y., Xiao L.Y., Chen Y.K., Wu P.C., Chen Y.H., Hu S.C, & Yuan S.S. (2020). Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo. ACS Omega, 5(12), 7018-7024.
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2

Multifunctional Nanoparticle Synthesis and Characterization

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Tetraethyl orthosilicate (TEOS), dopamine hydrochloride, anhydrous ethanol, ammonium hydroxide (NH3·H2O), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), and triphenylphosphine (TPP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Potassium permanganate (KMnO4, 99%), and sodium carbonate (Na2CO3), were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 2,2’-azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (AIBI) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Primary antibodies against Bcl-2 (Cat No. GTX100064), Bax (Cat No. GTX109683), Cytochrome c (Cyt-c, Cat No. GTX108585), cleaved-Caspase-9 (Cat No. GTX22324), cleaved-Caspase-3 (Cat No. GTX22302) and GAPDH (Cat No. GTX100118) were purchased from GeneTex (Irvine, CA, USA). All other chemicals and reagents were of the highest quality commercially available and used as received.
Hu H., Deng X., Song Q., Yang W., Zhang Y., Liu W., Wang S., Liang Z., Xing X., Zhu J., Zhang J., Shao Z., Wang B, & Zhang Y. (2021). Mitochondria-targeted accumulation of oxygen-irrelevant free radicals for enhanced synergistic low-temperature photothermal and thermodynamic therapy. Journal of Nanobiotechnology, 19, 390.
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