Purifications were performed using a Waters 2795 Separations Module (Waters Corporation) and an Acclaim 120 C18 HPLC column (4.6 mm by 150 mm, 5 μm; Thermo Fisher Scientific, Waltham, MA, USA) with a column oven temperature of 30°C and flow rate of 1 ml/min. For S1:10 purification, the mobile phase consisted of water with 0.1% (solvent A) and acetonitrile (solvent B). For in vivo S2:14 purification, two purification methods where solvent B consists of acetonitrile and methanol, respectively, were used in a sequential manner to enhance purity of compounds. The HPLC methods used for separating S1:10 and S2:14 are described in table S10. Fractions were collected using a 2211 Superrac Fraction Collector (LKB Bromma, Stockholm, Sweden).
Waters 2795 separations module
Manufactured by Waters Corporation
Sourced in United States
The Waters 2795 Separations Module is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative separations. It features a modular design, precise flow control, and advanced electronics to enable reliable and reproducible separations. The core function of the 2795 Separations Module is to facilitate the separation and purification of complex mixtures of chemical compounds.
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2 protocols using waters 2795 separations module
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HPLC Purification of Compounds S1:10 and S2:14
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HPLC Purification Methods for S1:10 and S2:14
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Purifications were performed using a Waters 2795 Separations Module (Waters Corporation) and an Acclaim 120 C18 HPLC column (4.6 mm × 150 mm, 5 µm; Thermo Fisher Scientific, Waltham, MA, USA) with a column oven temperature of 30°C and flow rate of 1 mL/min. For S1:10 purification, the mobile phase consisted of water with 0.1% (solvent A) and acetonitrile (solvent B). For in vivo S2:14 purification, two purification methods where solvent B consist of acetonitrile and methanol, respectively, were used in a sequential manner to enhance purity of compounds. The HPLC methods used for separating S1:10 and S2:14 are described in Table S10. Fractions were collected using a 2211 Superrac fraction collector (LKB Bromma, Stockholm, Sweden).
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